LOCUS       62056_4730       10510 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..82
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             83..790
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      816..1068
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1090..1114
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2415..3041
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3478..4542
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4611..4805
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5149..5289
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5475..6063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6153..6944)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7369..7393
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7471..7645)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7962..8510)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8533..8892)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(8987..9088)
                     /label=TRP1 promoter
     intron          10382..10497
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtatggact acaaagacca tgacggtgat tataaagatc atgacatcga
       61 ctacaaggat gacgatgaca agatggtgag caagggcgag gaggataaca tggccatcat
      121 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      181 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      241 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      301 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      361 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      421 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      481 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      541 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      601 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      661 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      721 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      781 gctgtacaag taaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
      841 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
      901 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
      961 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     1021 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     1081 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     1141 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     1201 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     1261 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     1321 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     1381 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     1441 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     1501 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     1561 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     1621 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     1681 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     1741 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     1801 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     1861 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     1921 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     1981 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     2041 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     2101 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     2161 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     2221 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     2281 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     2341 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     2401 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     2461 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     2521 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     2581 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     2641 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     2701 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     2761 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     2821 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     2881 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     2941 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     3001 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     3061 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     3121 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     3181 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     3241 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     3301 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     3361 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     3421 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     3481 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     3541 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     3601 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     3661 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     3721 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     3781 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     3841 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     3901 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     3961 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     4021 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     4081 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     4141 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     4201 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     4261 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     4321 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     4381 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     4441 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     4501 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     4561 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     4621 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     4681 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     4741 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     4801 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     4861 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     4921 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     4981 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     5041 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5101 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     5161 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     5221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5281 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5341 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6121 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
     6181 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
     6241 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
     6301 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
     6361 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
     6421 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
     6481 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
     6541 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
     6601 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
     6661 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
     6721 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
     6781 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
     6841 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
     6901 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
     6961 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
     7021 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
     7081 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
     7141 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     7201 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     7261 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     7321 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     7381 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     7441 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     7501 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     7561 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     7621 ctactcacac attattatgg agaaactcga ggcattgcag atgagctgta tctggaagag
     7681 gtaaacccga aacgcgtttt attcttgttg acatggagct attaaatcac tagaaggcac
     7741 tctttgctgc ttggacaaat gaacgtatct tatcgagatc ctgaacacca tttgtctcaa
     7801 ctccggagct gacatcgaca ccaacgatct tatatccaga ttcgtcaagc tgtttgatga
     7861 tttcagtaac gttaagtgga tcatgataat catcgcaaga ccggcaacag gattcaatct
     7921 taagaaactt tattgccaaa tgtttgaacg atctgcagtc agatctcggt gacgggcagg
     7981 accggacggg gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag
     8041 ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg
     8101 tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc
     8161 tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg
     8221 tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc
     8281 caggggcccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga
     8341 tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta
     8401 cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg
     8461 tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat ggttacttcc
     8521 taatcgaagc tttgcttggg tagaataggt aagtcagatt gaatctgaaa taaagggagg
     8581 aagggcgaac ttaagaaggt atgaccgggt cgttcactta ccttgcttga caaacgcacc
     8641 aagttatcgt gcaccaagca gcagatgata ataatgtcct cgttcctgtc tgctaataag
     8701 agtcacactt cgagcgccgc cgctactgct acaagtgggg ctgatctgac cagttgccta
     8761 aatgaaccat cttgtcaaac gacacaaatt ttgtgctcac cgcctggacg actaaaccaa
     8821 aataggcatt cattgttgac ctccactagc tccagccaag cccaaaaagt gctccttcaa
     8881 tatcatcttc tgtcgagtct agagcacatt ctgcggcctc tgtgctctgc aagccgcaaa
     8941 ctttcaccaa tggaccagaa ctacctgtga aattaataac agacatactc caagctgcct
     9001 ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca ccaatgccct ccctcttggc
     9061 cctctccttt tcttttttcg accgaattga gctcggtacc aaacgttgct agcagttcca
     9121 ggtggaatgt tatgatgagc attgtattaa atcaggagat atagcatgat ctctagttag
     9181 ctcaccacaa aagtcagacg gcgtaaccaa aagtcacaca acacaagctg taaggatttc
     9241 ggcacggcta cggaagacgg agaagccacc ttcagtggac tcgagtacca tttaattcta
     9301 tttgtgtttg atcgagacct aatacagccc ctacaacgac catcaaagtc gtatagctac
     9361 cagtgaggaa gtggactcaa atcgacttca gcaacatctc ctggataaac tttaagccta
     9421 aactatacag aataagatag gtggagagct tataccgagc tcccaaatct gtccagatca
     9481 tggttgaccg gtgcctggat cttcctatag aatcatcctt attcgttgac ctagctgatt
     9541 ctggagtgac ccagagggtc atgacttgag cctaaaatcc gccgcctcca ccatttgtag
     9601 aaaaatgtga cgaactcgtg agctctgtac agtgaccggt gactctttct ggcatgcgga
     9661 gagacggacg gacgcagaga gaagggctga gtaataagcc actggccaga cagctctggc
     9721 ggctctgagg tgcagtggat gattattaat ccgggaccgg ccgcccctcc gccccgaagt
     9781 ggaaaggctg gtgtgcccct cgttgaccaa gaatctattg catcatcgga gaatatggag
     9841 cttcatcgaa tcaccggcag taagcgaagg agaatgtgaa gccaggggtg tatagccgtc
     9901 ggcgaaatag catgccatta acctaggtac agaagtccaa ttgcttccga tctggtaaaa
     9961 gattcacgag atagtacctt ctccgaagta ggtagagcga gtacccggcg cgtaagctcc
    10021 ctaattggcc catccggcat ctgtagggcg tccaaatatc gtgcctctcc tgctttgccc
    10081 ggtgtatgaa accggaaagg ccgctcagga gctggccagc ggcgcagacc gggaacacaa
    10141 gctggcagtc gacccatccg gtgctctgca ctcgacctgc tgaggtccct cagtccctgg
    10201 taggcagctt tgccccgtct gtccgcccgg tgtgtcggcg gggttgacaa ggtcgttgcg
    10261 tcagtccaac atttgttgcc atattttcct gctctcccca ccagctgctc ttttcttttc
    10321 tctttctttt cccatcttca gtatattcat cttcccatcc aagaaccttt atttccccta
    10381 agtaagtact ttgctacatc catactccat ccttcccatc ccttattcct ttgaaccttt
    10441 cagttcgagc tttcccactt catcgcagct tgactaacag ctaccccgct tgagcagaca
    10501 tcaccatggt
//
