LOCUS       62056_4725       11003 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             60..83
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             84..800
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      826..1078
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1100..1124
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2425..3051
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3488..4552
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4621..4815
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5159..5299
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5485..6073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6163..6954)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7379..7403
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7481..7655)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7981..9003)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9026..9385)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(9480..9581)
                     /label=TRP1 promoter
     intron          10875..10990
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtcatggac tacaaagacc atgacggtga ttataaagat catgacatcg
       61 actacaagga tgacgatgac aagatggtga gcaagggcga ggagctgttc accggggtgg
      121 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      181 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      241 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      301 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      361 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      421 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      481 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      541 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      601 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      661 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
      721 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      781 gcatggacga gctgtacaag taaggtgacc agctcgaatt tccccgatcg ttcaaacatt
      841 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
      901 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
      961 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     1021 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     1081 gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     1141 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     1201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     1261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     1321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     1381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     1441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     1501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     1561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     1621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     1681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     1741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     2041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     2101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     2161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     2221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     2281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     2341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     2401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     2461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     2521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     2581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     2641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     2701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     2761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     3001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     3061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     3121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     3181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     3241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     3301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     3361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     3421 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     3481 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     3541 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     3601 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     3661 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     3721 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     3781 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     3841 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     3901 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     3961 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     4021 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     4081 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     4141 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     4201 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     4261 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     4321 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     4381 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     4441 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     4501 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     4561 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     4621 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     4681 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     4741 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     4801 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     4861 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     4921 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     4981 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     5041 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5101 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     5161 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     5221 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5281 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5341 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5461 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5521 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5581 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5641 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5701 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5761 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5821 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5881 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5941 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6001 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6061 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6121 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     6181 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     6241 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     6301 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     6361 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     6421 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     6481 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     6541 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     6601 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     6661 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     6721 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     6781 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     6841 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     6901 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     6961 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     7021 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     7081 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     7141 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     7201 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     7261 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     7321 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     7381 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     7441 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
     7501 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     7561 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     7621 tatctgggaa ctactcacac attattatgg agaaactcga ggcattgcag atgagctgta
     7681 tctggaagag gtaaacccga aacgcgtttt attcttgttg acatggagct attaaatcac
     7741 tagaaggcac tctttgctgc ttggacaaat gaacgtatct tatcgagatc ctgaacacca
     7801 tttgtctcaa ctccggagct gacatcgaca ccaacgatct tatatccaga ttcgtcaagc
     7861 tgtttgatga tttcagtaac gttaagtgga tcatgataat catcgcaaga ccggcaacag
     7921 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcaggt cgacggacta
     7981 tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
     8041 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
     8101 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
     8161 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
     8221 tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
     8281 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
     8341 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     8401 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     8461 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     8521 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     8581 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     8641 cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc
     8701 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctaaactc
     8761 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     8821 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     8881 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     8941 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     9001 catggttact tcctaatcga agctttgctt gggtagaata ggtaagtcag attgaatctg
     9061 aaataaaggg aggaagggcg aacttaagaa ggtatgaccg ggtcgttcac ttaccttgct
     9121 tgacaaacgc accaagttat cgtgcaccaa gcagcagatg ataataatgt cctcgttcct
     9181 gtctgctaat aagagtcaca cttcgagcgc cgccgctact gctacaagtg gggctgatct
     9241 gaccagttgc ctaaatgaac catcttgtca aacgacacaa attttgtgct caccgcctgg
     9301 acgactaaac caaaataggc attcattgtt gacctccact agctccagcc aagcccaaaa
     9361 agtgctcctt caatatcatc ttctgtcgag tctagagcac attctgcggc ctctgtgctc
     9421 tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat aacagacata
     9481 ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc
     9541 cctccctctt ggccctctcc ttttcttttt tcgaccgaat tgagctcggt accaaacgtt
     9601 gctagcagtt ccaggtggaa tgttatgatg agcattgtat taaatcagga gatatagcat
     9661 gatctctagt tagctcacca caaaagtcag acggcgtaac caaaagtcac acaacacaag
     9721 ctgtaaggat ttcggcacgg ctacggaaga cggagaagcc accttcagtg gactcgagta
     9781 ccatttaatt ctatttgtgt ttgatcgaga cctaatacag cccctacaac gaccatcaaa
     9841 gtcgtatagc taccagtgag gaagtggact caaatcgact tcagcaacat ctcctggata
     9901 aactttaagc ctaaactata cagaataaga taggtggaga gcttataccg agctcccaaa
     9961 tctgtccaga tcatggttga ccggtgcctg gatcttccta tagaatcatc cttattcgtt
    10021 gacctagctg attctggagt gacccagagg gtcatgactt gagcctaaaa tccgccgcct
    10081 ccaccatttg tagaaaaatg tgacgaactc gtgagctctg tacagtgacc ggtgactctt
    10141 tctggcatgc ggagagacgg acggacgcag agagaagggc tgagtaataa gccactggcc
    10201 agacagctct ggcggctctg aggtgcagtg gatgattatt aatccgggac cggccgcccc
    10261 tccgccccga agtggaaagg ctggtgtgcc cctcgttgac caagaatcta ttgcatcatc
    10321 ggagaatatg gagcttcatc gaatcaccgg cagtaagcga aggagaatgt gaagccaggg
    10381 gtgtatagcc gtcggcgaaa tagcatgcca ttaacctagg tacagaagtc caattgcttc
    10441 cgatctggta aaagattcac gagatagtac cttctccgaa gtaggtagag cgagtacccg
    10501 gcgcgtaagc tccctaattg gcccatccgg catctgtagg gcgtccaaat atcgtgcctc
    10561 tcctgctttg cccggtgtat gaaaccggaa aggccgctca ggagctggcc agcggcgcag
    10621 accgggaaca caagctggca gtcgacccat ccggtgctct gcactcgacc tgctgaggtc
    10681 cctcagtccc tggtaggcag ctttgccccg tctgtccgcc cggtgtgtcg gcggggttga
    10741 caaggtcgtt gcgtcagtcc aacatttgtt gccatatttt cctgctctcc ccaccagctg
    10801 ctcttttctt ttctctttct tttcccatct tcagtatatt catcttccca tccaagaacc
    10861 tttatttccc ctaagtaagt actttgctac atccatactc catccttccc atcccttatt
    10921 cctttgaacc tttcagttcg agctttccca cttcatcgca gcttgactaa cagctacccc
    10981 gcttgagcag acatcaccat ggt
//
