LOCUS       Exported               11849 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11849)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(1081..11849,1..1080)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    137..158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        173..203
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     235..251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             252..479
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    260..316
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(320..336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        713..1058
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          1090..1279
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1295..3097
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="RPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAV
                     PGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVME
                     HQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFF
                     NYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVV
                     ATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLI
                     NHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWAD
                     EHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKN
                     HPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLF
                     DVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTD
                     MWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPK
                     SAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             3104..3121
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3156..3408
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3430..3454
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4755..5384
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5813..6886
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6952..7146
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7490..7630
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7816..8404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8491..9285)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9710..9734
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9812..9986
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10027..11052)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11119..11795)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
       61 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      121 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagctatga catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct
      301 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
      481 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa
      541 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
      601 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
      661 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
      721 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
      781 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
      841 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
      901 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
      961 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     1021 cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt gaccatggta
     1081 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
     1141 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
     1201 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
     1261 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
     1321 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
     1381 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
     1441 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
     1501 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
     1561 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
     1621 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
     1681 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
     1741 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
     1801 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
     1861 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
     1921 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
     1981 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
     2041 actgtgcgtc acagccaaaa gccagacaga gtgtgatatc tacccgcttc gcgtcggcat
     2101 ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt tctactttac
     2161 tggctttggt cgtcatgaag atgcggactt gcgtggcaaa ggattcgata acgtgctgat
     2221 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
     2281 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
     2341 aactgctgct gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc
     2401 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
     2461 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
     2521 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     2581 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     2641 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     2701 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     2761 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     2821 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     2881 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     2941 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     3001 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     3061 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
     3121 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     3181 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     3241 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     3301 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     3361 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     3421 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     3481 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     3541 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     3601 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     3661 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     3721 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     3781 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     3841 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     3901 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     3961 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     4021 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     4081 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     4141 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     4201 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     4261 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     4321 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     4381 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     4441 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     4501 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     4561 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     4621 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     4681 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     4741 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     4801 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     4861 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     4921 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     4981 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     5041 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     5101 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     5161 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     5221 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     5281 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     5341 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     5401 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     5461 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     5521 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     5581 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     5641 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     5701 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     5761 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     5821 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     5881 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     5941 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     6001 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     6061 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     6121 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     6181 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     6241 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     6301 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     6361 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     6421 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     6481 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     6541 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     6601 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     6661 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     6721 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     6781 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     6841 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     6901 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     6961 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     7021 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     7081 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     7141 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     7201 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     7261 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     7321 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     7381 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7441 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7501 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     7561 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7621 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7681 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7741 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8221 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8461 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
     8521 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
     8581 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
     8641 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
     8701 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
     8761 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
     8821 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
     8881 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
     8941 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
     9001 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
     9061 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
     9121 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
     9181 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
     9241 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
     9301 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     9361 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     9421 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     9481 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     9541 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     9601 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     9661 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     9721 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
     9781 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
     9841 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
     9901 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
     9961 actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca
    10021 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
    10081 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
    10141 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
    10201 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
    10261 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
    10321 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
    10381 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
    10441 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
    10501 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
    10561 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
    10621 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
    10681 cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc
    10741 aggcaggtct tgcaacgtga caccctgtga acggcgggag atgcaatagg tcaggctctc
    10801 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
    10861 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
    10921 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
    10981 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
    11041 aggctttttc atatctcatt gccccccgga tctgcgaaag ctcgagagag atagatttgt
    11101 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
    11161 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
    11221 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    11281 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
    11341 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
    11401 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
    11461 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    11521 gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa
    11581 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
    11641 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
    11701 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
    11761 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
    11821 tatctttgat attcttggag tagacgaga
//
