LOCUS       62056_4720       12813 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          10..199
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             194..2017
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2024..2041
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2076..2328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2350..2374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3674..4300
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4737..5801
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5870..6064
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6408..6548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6734..7322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7412..8203)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8628..8652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8730..8904)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8947..9738)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(9806..10483)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10674..10695
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10710..10740
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10748..10764
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10772..10788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10826..11078)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     11149..11165
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(11193..11538)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        12446..12791
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
       61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
      121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
      181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
      241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
      301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
      361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
      421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
      481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
      541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
      601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
      661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
      721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
      781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
      841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
      901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
      961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
     1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac
     1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
     1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
     1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
     1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
     1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
     1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
     1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
     2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
     4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     5041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     5101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     5161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     5221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     5281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     5341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     5401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     5461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     5521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     5581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     5641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     5701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     5761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     5821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     5881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     5941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     6001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
     7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
     8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
     8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
     8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
     8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct
     8941 acttcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     9001 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     9061 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     9121 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     9181 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     9241 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     9301 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     9361 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     9421 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     9481 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     9541 ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     9601 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     9661 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     9721 tccatcttgt tcaatcatat ctcattgccc cccgggatct gcgaaagctc gagagagata
     9781 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
     9841 atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     9901 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     9961 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    10021 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    10081 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    10141 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    10201 gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
    10261 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    10321 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
    10381 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
    10441 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    10501 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
    10561 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10621 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10681 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10741 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    10801 tctcatgttt gacagcttat catcggatct agtaacatag atgacaccgc gcgcgataat
    10861 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
    10921 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
    10981 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
    11041 aatcttaaga aactttattg ccaaatgttt gaacgatctg cagcccgggg gatccactag
    11101 ttctagagcg gccgccaccg cggtggagct cggtaccggg ccccccctcg aggtcgacgg
    11161 tatcgataag cttgataaac tagagtcccc cgtgttctct ccaaatgaaa tgaacttcct
    11221 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    11281 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    11341 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg
    11401 atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc
    11461 ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc
    11521 ctttgttgaa aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg
    11581 gagtagacaa gtgtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt
    11641 cgtaagagac tctgtatgaa ctgttcgcca gtctttacgg cgagttctgt taggtcctct
    11701 atttgaatct ttgactccat ggcctttgat tcagtgggaa ctaccttttt agagactcca
    11761 atctctatta cttgccttgg tttgtgaagc aagccttgaa tcgtccatac tggaatagta
    11821 cttctgatct tgagaaatat atctttctct gtgttcttga tgcagttagt cctgaatctt
    11881 ttgactgcat ctttaacctt cttgggaagg tatttgattt cctggagatt attgctcggg
    11941 tagatcgtct tgatgagacc tgctgcgtaa gcctctctaa ccatctgtgg gttagcattc
    12001 tttctgaaat tgaaaaggct aatctgggga cctgcaggca tgcaagcttg gcactggccg
    12061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    12121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    12181 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
    12241 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
    12301 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
    12361 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
    12421 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
    12481 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
    12541 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
    12601 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    12661 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    12721 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    12781 tggagagaac acgggggact cttgaccatg gta
//
