LOCUS       62056_4715       11745 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             373..1089
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="LTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1096..1113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1145..1397
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1419..1443
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2743..3369
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3806..4870
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4939..5133
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5477..5617
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5803..6391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6481..7272)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7697..7721
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7799..7973)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8016..9038)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9106..9783)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9974..9995
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10010..10040
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10048..10064
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10072..10088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10126..10378)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     10449..10465
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10493..10838)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
      361 cccatggtag atctgactag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagg ctagccacca ccaccaccac cacgtgtgaa ttggtgacca gctcgaattt
     1141 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     1201 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     1261 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     1321 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     1381 atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta
     1441 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
     1501 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     1561 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     1621 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     1681 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     1741 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     1801 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     1861 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     1921 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     1981 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     2041 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     2101 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     2161 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     2221 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     2281 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     2341 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     2401 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     2461 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     2521 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     2581 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     2641 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     2701 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     2761 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     2821 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     2881 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     2941 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     3001 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     3061 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     3121 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     3181 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     3241 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     3301 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     3361 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     3421 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     3481 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     3541 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     3601 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     3661 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     3721 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     3781 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
     3841 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     3901 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     3961 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     4021 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     4081 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     4141 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     4201 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     4261 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     4321 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     4381 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     4441 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     4501 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     4561 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     4621 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     4681 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     4741 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     4801 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     4861 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     4921 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     4981 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     5041 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     5101 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     5161 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     5221 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     5281 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     5341 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5401 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5461 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     5521 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5581 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     5641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6241 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta
     6481 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
     6541 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
     6601 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
     6661 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
     6721 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
     6781 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
     6841 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
     6901 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
     6961 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
     7021 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
     7081 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
     7141 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
     7201 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
     7261 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
     7321 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
     7381 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
     7441 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     7501 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     7561 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     7621 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     7681 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     7741 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
     7801 tctggatttt agtactggat tttggtttta ggaattagaa attttattga tagaagtatt
     7861 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
     7921 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
     7981 ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg
     8041 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     8101 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     8161 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     8221 tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga
     8281 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     8341 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     8401 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     8461 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     8521 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     8581 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     8641 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt
     8701 tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     8761 cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc
     8821 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     8881 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     8941 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     9001 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct
     9061 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     9121 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
     9181 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     9241 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     9301 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     9361 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     9421 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     9481 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
     9541 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     9601 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     9661 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     9721 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     9781 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     9841 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
     9901 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9961 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10021 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10081 gctatgacat gattacgaat tctcatgttt gacagcttat catcggatct agtaacatag
    10141 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
    10201 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
    10261 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
    10321 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatctg
    10381 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct cggtaccggg
    10441 ccccccctcg aggtcgacgg tatcgataag cttgataaac tagagtcccc cgtgttctct
    10501 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
    10561 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
    10621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    10681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
    10741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
    10801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
    10861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacgaaga
    10921 ttttcttctt gtcattgagt cgtaagagac tctgtatgaa ctgttcgcca gtctttacgg
    10981 cgagttctgt taggtcctct atttgaatct ttgactccat ggcctttgat tcagtgggaa
    11041 ctaccttttt agagactcca atctctatta cttgccttgg tttgtgaagc aagccttgaa
    11101 tcgtccatac tggaatagta cttctgatct tgagaaatat atctttctct gtgttcttga
    11161 tgcagttagt cctgaatctt ttgactgcat ctttaacctt cttgggaagg tatttgattt
    11221 cctggagatt attgctcggg tagatcgtct tgatgagacc tgctgcgtaa gcctctctaa
    11281 ccatctgtgg gttagcattc tttctgaaat tgaaaaggct aatctgggga cctgcaggca
    11341 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    11401 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11461 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
    11521 gagcttggat cagattgtcg tttcccgcct tcagtttagc ttcatggagt caaagattca
    11581 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
    11641 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
    11701 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaat
//
