LOCUS       62056_4690       11753 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2206..2847
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    3074..3098
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4398..5024
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5461..6525
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6594..6788
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7132..7272
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7458..8046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8136..8927)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9352..9376
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9454..9628)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9671..10693)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10761..11438)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11629..11650
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11665..11695
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11703..11719
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11727..11743
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagtg
      901 tcgaccagat ctcgtacgcg tggaggagga ggaagcggag gaggaggaag cgaagacgcc
      961 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1021 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1081 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1141 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1201 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     1261 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     1321 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     1381 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     1441 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     1501 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     1561 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     1621 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1681 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1741 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     1801 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     1861 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     1921 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     1981 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2041 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2101 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2161 ccggaagcga ccaacgcctt gattgacaag gatggatgac tgcagagctt tcgttcgtat
     2221 catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga
     2281 atcctactga gttcgagtat tatggcattg ggaaacatgt ttttcttgta ccatttgttg
     2341 tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg
     2401 gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt
     2461 gttttttctc ttatttgttg tgtgttgaat ttgaaaatat aagagatatg caaacatttt
     2521 gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta
     2581 aaacacttgt agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct
     2641 agaaaagctg caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga
     2701 actttccttt atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta
     2761 tagttatact catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact
     2821 tgccaattga ttgacaacat gcatcaagct tggcactggc cgtcgtttta caacgtcgtg
     2881 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2941 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3001 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     3061 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     3121 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     3181 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     3241 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     3301 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     3361 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     3421 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     3481 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     3541 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     3601 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     3661 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3721 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3781 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3841 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3901 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3961 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     4021 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     4081 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     4141 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     4201 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     4261 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     4321 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     4381 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     4441 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     4501 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     4561 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     4621 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     4681 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4741 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4801 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4861 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4921 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4981 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     5041 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     5101 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     5161 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     5221 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     5281 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     5341 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     5401 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     5461 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5521 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5581 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5641 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5701 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5761 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5821 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5881 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5941 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     6001 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     6061 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     6121 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     6181 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     6241 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     6301 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     6361 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6421 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6481 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6541 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     6601 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6661 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6721 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6781 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6841 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6901 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6961 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     7021 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7141 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7201 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7261 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7861 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8101 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     8161 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     8221 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     8281 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     8341 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     8401 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     8461 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     8521 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     8581 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     8641 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     8701 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     8761 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     8821 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     8881 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     8941 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     9001 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     9061 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     9121 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     9181 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     9241 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     9301 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     9361 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     9421 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     9481 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     9541 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     9601 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
     9661 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     9721 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     9781 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     9841 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     9901 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     9961 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
    10021 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
    10081 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
    10141 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
    10201 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
    10261 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
    10321 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
    10381 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
    10441 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
    10501 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
    10561 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
    10621 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
    10681 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
    10741 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    10801 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    10861 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    10921 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    10981 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    11041 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    11101 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    11161 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
    11221 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    11281 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    11341 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    11401 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    11461 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
    11521 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    11581 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    11641 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    11701 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acg
//
