LOCUS       Exported               11183 bp DNA     circular SYN 16-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11183)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11183)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11183)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    220..244
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1545..2171
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2609..3673
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3742..3936
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4280..4420
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4606..5194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5284..6075)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6500..6524
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6602..6776)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6820..7842)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7909..8585)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8776..8797
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8812..8842
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        9399..9744
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9751..9802
                     /label=MCS
                     /note="multiple cloning site"
     CDS             9805..10512
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      10535..11176
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      121 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
      181 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
      241 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
      301 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
      361 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
      421 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
      481 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
      541 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
      601 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
      661 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
      721 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
      781 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
      841 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
      901 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
      961 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     1021 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     1081 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     1141 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     1201 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     1261 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     1321 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     1381 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     1441 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     1501 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     1561 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     1621 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     1681 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     1741 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     1801 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     1861 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     1921 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     1981 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     2041 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     2101 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     2161 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     2221 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     2281 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     2341 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     2401 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     2461 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     2521 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     2581 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     2641 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     2701 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     2761 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     2821 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     2881 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     2941 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     3001 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     3061 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     3121 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     3181 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     3241 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     3301 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     3361 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     3421 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     3481 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     3541 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     3601 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     3661 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     3721 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     3781 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     3841 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     3901 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     3961 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     4021 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     4081 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     4141 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4261 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     4321 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4381 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     4441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5041 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     5281 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     5341 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     5401 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     5461 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     5521 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     5581 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     5641 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     5701 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     5761 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     5821 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     5881 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     5941 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     6001 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     6061 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     6121 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     6181 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     6241 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     6301 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     6361 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     6421 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     6481 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     6541 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     6601 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     6661 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     6721 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     6781 agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt
     6841 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     6901 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     6961 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     7021 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
     7081 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     7141 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     7201 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     7261 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     7321 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     7381 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     7441 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
     7501 cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga
     7561 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
     7621 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     7681 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     7741 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     7801 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga
     7861 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     7921 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     7981 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8041 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8101 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8161 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8221 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8281 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     8341 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     8401 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     8461 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     8521 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     8581 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     8641 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
     8701 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8761 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8821 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8881 cagctatgac atgattacga attcggtccc cagattagcc ttttcaattt cagaaagaat
     8941 gctaacccac agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg
     9001 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     9061 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
     9121 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
     9181 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
     9241 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
     9301 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
     9361 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
     9421 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
     9481 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
     9541 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     9601 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     9661 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     9721 agttcatttc atttggagag aacacggggg acgagctcaa gcttcgaatt ctgcagtcga
     9781 cggtaccgcg ggcccgggat cctgatggtg agcaagggcg aggaggataa catggccatc
     9841 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     9901 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     9961 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
    10021 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
    10081 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
    10141 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
    10201 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
    10261 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
    10321 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
    10381 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
    10441 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
    10501 gagctgtaca agtgatctag gggctagaat gcagagcttt cgttcgtatc atcggtttcg
    10561 acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag
    10621 tttgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt
    10681 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga
    10741 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct
    10801 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa
    10861 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta
    10921 gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc
    10981 aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta
    11041 tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat agttatactc
    11101 atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt gccaattgat
    11161 tgacaacatg catcaagctt ggc
//
