LOCUS       Exported               10989 bp DNA     circular SYN 21-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1442..2083
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2310..2334
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3635..4261
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4699..5763
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5832..6026
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6370..6510
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6696..7284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7374..8165)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8590..8614
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8692..8866)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8910..9932)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9999..10675)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10866..10887
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10902..10932
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10940..10956
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10964..10980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataaag aagttcattt catttggaga
      841 gaacacgggg gacgagctca tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
      901 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
      961 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     1021 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     1081 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     1141 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     1201 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     1261 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtggg
     1321 aggaggaagc ggaggaggaa gcggaggagg aagcggagga ggaagcagat ctcgtacgcg
     1381 tcccggggcg gtacccggga tcctctagag ggcccggcgc gccactagtg tcgacctgca
     1441 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
     1501 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
     1561 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
     1621 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
     1681 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
     1741 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
     1801 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
     1861 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
     1921 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
     1981 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
     2041 aatgcatttt atgacttgcc aattgattga caacatgcat caagcttggc actggccgtc
     2101 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2161 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2221 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
     2281 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     2341 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     2401 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     2461 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     2521 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     2581 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     2641 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     2701 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     2761 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     2821 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     2881 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     2941 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     3001 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     3061 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     3121 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     3181 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     3241 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     3301 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     3361 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     3421 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     3481 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     3541 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     3601 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     3661 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     3721 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     3781 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     3841 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     3901 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     3961 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     4021 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     4081 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     4141 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     4201 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     4261 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     4321 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     4381 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     4441 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     4501 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     4561 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     4621 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
     4681 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     4741 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     4801 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     4861 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     4921 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     4981 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5041 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5101 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5161 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5221 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5281 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5341 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5401 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     5461 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     5521 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     5581 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     5641 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     5701 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     5761 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     5821 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     5881 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     5941 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6001 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6061 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6121 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6181 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6361 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6481 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7321 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     7381 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     7441 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     7501 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     7561 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     7621 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     7681 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     7741 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     7801 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     7861 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     7921 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     7981 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     8041 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     8101 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     8161 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     8221 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     8281 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     8341 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     8401 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     8461 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     8521 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     8581 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     8641 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     8701 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     8761 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     8821 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     8881 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     8941 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     9001 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     9061 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     9121 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     9181 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     9241 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     9301 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     9361 acttcggggc agtcctcggc ccaaagcatc agatcatcga gagcctgcgc gacggacgca
     9421 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     9481 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     9541 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     9601 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     9661 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     9721 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     9781 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     9841 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     9901 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag
     9961 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
    10021 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
    10081 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    10141 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    10201 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    10261 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    10321 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
    10381 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg
    10441 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    10501 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    10561 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    10621 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    10681 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    10741 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
    10801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10861 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10921 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10981 atgattacg
//
