LOCUS       40924_8871       10130 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA1300-FAD2b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Xie JX, Cai NB, Zhuang WJ.
  TITLE     Use of anti-RNA technology to improve the quality of peanut oil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   Zhuang WJ, Xie JX, Cai NB.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2006) Crop Science Department, Fujian Agriculture 
            and Forestry University, Oil Crop Institution, Jinshan, Fuzhou, 
            Fujian 350002, P.R. China
REFERENCE   3  (bases 1 to 10130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAY-2006) Crop Science Department, Fujian Agriculture and 
            Forestry University, Oil Crop Institution, Jinshan, Fuzhou, Fujian 
            350002, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(8..260)
                     /label=NOS
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      8..260
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(312..875)
                     /note="reverse DNA sequence of partial peanut delta-12
                     fatty acid desaturase gene (fad)"
     regulatory      complement(882..1191)
                     /label=soybean oleosin promoter
                     /note="soybean oleosin promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1232..1248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1451..1475
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2775..3401
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3838..4902
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4971..5165
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5509..5649
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5835..6423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6513..7304)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7729..7753
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7831..8005)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8048..9070)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(9138..9815)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10006..10027
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10042..10072
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10080..10096
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10104..10120
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gagctctcta cattggtaac aggagctttg
      301 acaacacaac aatgggagct ggagggcgtg tcactaagat tgaagctcaa gagaagcctc
      361 tttcaagggt tccacattca aaccctccat tcagtgttgg ccaactcaag aaagcaattc
      421 caccacattg ctttgaacgt tctcttttca tatcattctc ctatgttgtc tatgatctct
      481 taatggccta cttactcttc tacattgcca ccacttattt ccaccagctt ccatacccat
      541 tttccttcct tgcttggcca atctattggg ccatccaagg ctgcattctc actggtgttt
      601 gggtgattgc tcatgagtgt ggccaccatg ccttcagcaa gtatcaactt gttgatgaca
      661 tggttgggtt tgatccttca ctcttgtcta ttagtccctt atttctcatg gaaaatcagc
      721 caccgccacc tccactccaa caccggttcc ctcgaccgcg actaagtgtt tgtcctgaaa
      781 ccaaaatcaa aggtatcacg gtataacaag tacatgaaca atccactaga gagggctatt
      841 tcccttttca tcacactcac actaggatgc cctggcccgg gggttgaagg tgaagtttag
      901 ggttttgcaa tgaagatgat gatgcaacag tgctgcaagt gaaggaattg ttagtggggt
      961 gtgtgttaga gagggagagt gtgacggggg tgttgttata tagcaaaagt ggagttgttg
     1021 ggtgatgaca cgtacatgat acatggtgag taggggcttt gcatgaggaa caggtttcag
     1081 tttgccctcg gtttagtaca tcagaaaaat taaagtgggg tacataaatt atgacagaca
     1141 aatgagaata atatactttt taacattttt attaaggaca gttgggatac agggatcctc
     1201 tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact
     1261 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1321 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1381 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     1441 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     1501 ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     1561 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     1621 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     1681 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     1741 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     1801 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     1861 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     1921 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     1981 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     2041 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     2101 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     2161 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     2221 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     2281 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     2341 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     2401 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     2461 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     2521 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     2581 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     2641 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     2701 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     2761 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     2821 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     2881 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     2941 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     3001 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     3061 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     3121 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     3181 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     3241 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     3301 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     3361 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     3421 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     3481 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     3541 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     3601 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     3661 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     3721 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     3781 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     3841 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     3901 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     3961 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     4021 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     4081 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     4141 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     4201 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     4261 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     4321 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     4381 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     4441 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     4501 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     4561 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     4621 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     4681 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     4741 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     4801 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     4861 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     4921 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     4981 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     5041 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     5101 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     5161 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     5221 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     5281 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     5341 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     5401 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5461 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     5521 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     5581 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5641 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6241 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6481 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
     6541 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
     6601 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
     6661 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
     6721 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
     6781 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
     6841 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
     6901 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
     6961 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
     7021 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
     7081 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
     7141 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
     7201 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
     7261 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
     7321 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
     7381 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
     7441 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
     7501 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     7561 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     7621 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     7681 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     7741 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     7801 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     7861 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     7921 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     7981 ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc
     8041 tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     8101 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     8161 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     8221 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     8281 cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     8341 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     8401 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     8461 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     8521 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     8581 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     8641 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     8701 cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag
     8761 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     8821 taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     8881 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     8941 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     9001 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     9061 gctttttcat atctcattgc cccccgggat ctgcgaaagc tcgagagaga tagatttgta
     9121 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     9181 gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     9241 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     9301 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     9361 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     9421 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     9481 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     9541 gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
     9601 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     9661 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     9721 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     9781 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     9841 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct
     9901 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9961 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    10021 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    10081 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
//
