LOCUS       40924_8866       10407 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA1300-FAD2A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Xie JX, Cai NB, Zhuang WJ.
  TITLE     Use of anti-RNA technology to improve the quality of peanut oil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Zhuang WJ, Xie JX, Cai NB.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2006) Crop Science Department, Fujian Agriculture 
            and Forestry University, Oil Crop Institution, Jinshan, Fuzhou, 
            Fujian 350002, P.R. China
REFERENCE   3  (bases 1 to 10407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAY-2006) Crop Science Department, Fujian Agriculture and 
            Forestry University, Oil Crop Institution, Jinshan, Fuzhou, Fujian 
            350002, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(312..875)
                     /note="reverse DNA sequence of partial peanut delta-12
                     fatty acid desaturase gene (fad)"
     regulatory      complement(882..1468)
                     /label=soybean oleosin promoter
                     /note="soybean oleosin promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1509..1525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1728..1752
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3052..3678
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4115..5179
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5248..5442
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5786..5926
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6112..6700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6790..7581)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8006..8030
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8108..8282)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8325..9347)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(9415..10092)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10283..10304
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10319..10349
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10357..10373
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10381..10397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gagctctcta cattggtaac aggagctttg
      301 acaacacaac aatgggagct ggagggcgtg tcactaagat tgaagctcaa gagaagcctc
      361 tttcaagggt tccacattca aaccctccat tcagtgttgg ccaactcaag aaagcaattc
      421 caccacattg ctttgaacgt tctcttttca tatcattctc ctatgttgtc tatgatctct
      481 taatggccta cttactcttc tacattgcca ccacttattt ccaccagctt ccatacccat
      541 tttccttcct tgcttggcca atctattggg ccatccaagg ctgcattctc actggtgttt
      601 gggtgattgc tcatgagtgt ggccaccatg ccttcagcaa gtatcaactt gttgatgaca
      661 tggttgggtt tgatccttca ctcttgtcta ttagtccctt atttctcatg gaaaatcagc
      721 caccgccacc tccactccaa caccggttcc ctcgaccgcg actaagtgtt tgtcctgaaa
      781 ccaaaatcaa aggtatcacg gtataacaag tacatgaaca atccactaga gagggctatt
      841 tcccttttca tcacactcac actaggatgc cctggcccgg gggttgaagg tgaagtttag
      901 ggttttgcaa tgaagatgat gatgcaacag tgctgcaagt gaaggaattg ttagtggggt
      961 gtgtgttaga gagggagagt gtgacggggg tgttgttata tagcaaaagt ggagttgttg
     1021 ggtgatgaca cgtacatgat acatggtgag taggggcttt gcatgaggaa caggtttcag
     1081 tttgccctcg gtttagtaca tcagaaaaat taaagtgggg tacataaatt atgacagaca
     1141 aatgagaata atatactttt taacattttt attaaggaca gttgggatac agtttcgcag
     1201 gtggtgttgg tggtattttt catcataatt ggagttttac ttcacaaaat ctgcattagc
     1261 atttaatgaa attagtttgt aatccgtgca tcgtttgtaa atttttttgc atatatattt
     1321 atttgttttg taaagtaaaa ataaaatgaa aatttatgtg caaattaaaa tagattaaac
     1381 attgataata tatttacata gtagataaat tgttcagaaa agcaagaaag ataaacacta
     1441 ttatcaagaa gtatcagttt aacattcccg gatcctctag agtcgacctg caggcatgca
     1501 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     1561 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     1621 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     1681 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     1741 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     1801 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     1861 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     1921 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     1981 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     2041 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     2101 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     2161 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     2221 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     2281 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     2341 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     2401 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     2461 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     2521 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     2581 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     2641 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     2701 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     2761 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     2821 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     2881 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     2941 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     3001 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     3061 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     3121 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     3181 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     3241 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     3301 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     3361 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     3421 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     3481 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     3541 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     3601 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     3661 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     3721 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     3781 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     3841 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     3901 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     3961 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     4021 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     4081 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     4141 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     4201 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     4261 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     4321 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     4381 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     4441 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     4501 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     4561 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     4621 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     4681 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     4741 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     4801 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     4861 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     4921 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     4981 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     5041 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     5101 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     5161 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     5221 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     5281 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     5341 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     5401 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     5461 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     5521 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     5581 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     5641 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     5701 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     5761 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     5821 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5881 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     5941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6541 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     6781 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
     6841 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
     6901 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
     6961 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
     7021 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
     7081 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
     7141 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
     7201 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
     7261 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
     7321 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
     7381 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
     7441 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
     7501 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
     7561 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
     7621 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
     7681 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
     7741 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
     7801 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     7861 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     7921 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     7981 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     8041 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     8101 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     8161 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     8221 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     8281 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
     8341 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     8401 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     8461 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     8521 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
     8581 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     8641 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     8701 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     8761 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     8821 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     8881 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     8941 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
     9001 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     9061 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
     9121 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     9181 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     9241 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     9301 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
     9361 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
     9421 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
     9481 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     9541 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     9601 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     9661 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     9721 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     9781 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
     9841 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     9901 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     9961 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    10021 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    10081 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    10141 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc
    10201 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10261 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10321 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10381 caggaaacag ctatgaccat gattacg
//
