LOCUS       62056_4680       11017 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             860..1576
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1701..2342
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2569..2593
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3893..4519
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4956..6020
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6089..6283
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6627..6767
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6953..7541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7631..8422)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8847..8871
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8949..9123)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9166..9954)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(10025..10702)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10893..10914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10929..10959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10967..10983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10991..11007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataaag aagttcattt catttggaga
      841 gaacacgggg gacgagctca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      961 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1261 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1321 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1381 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1441 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1501 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1561 ggacgagctg tacaagaaga agggcggaaa gatcgccgtg ggaggtggct catctggcgg
     1621 aggtcagatc tcgtacgcgt cccggggcgg tacccgggat cctctagagg gcccggcgcg
     1681 ccactagtgt cgacctgcag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa
     1741 gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagttcg agtattatgg
     1801 cattgggaaa catgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta
     1861 ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata
     1921 tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt
     1981 tgaatttgaa aatataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc
     2041 gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg
     2101 cttattcact aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat
     2161 acaagtatgt cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga
     2221 atccttgtca gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt
     2281 gagtatgaaa atatttttta atgcatttta tgacttgcca attgattgac aacatgcatc
     2341 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2401 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2461 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     2521 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     2581 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     2641 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     2701 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     2761 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     2821 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     2881 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     2941 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     3001 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     3061 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     3121 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     3181 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     3241 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     3301 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     3361 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     3421 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     3481 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     3541 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     3601 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     3661 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     3721 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     3781 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     3841 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     3901 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     3961 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     4021 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     4081 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     4141 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     4201 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     4261 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     4321 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     4381 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     4441 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     4501 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     4561 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     4621 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     4681 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     4741 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     4801 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     4861 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     4921 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     4981 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     5041 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5101 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5161 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5221 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5281 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5341 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5401 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5461 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     5521 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     5581 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     5641 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     5701 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     5761 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     5821 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     5881 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     5941 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6001 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6061 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6121 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6181 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6241 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6301 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6361 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6421 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6481 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6541 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6601 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6661 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6721 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     6781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7381 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
     7621 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     7681 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     7741 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     7801 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     7861 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     7921 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     7981 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     8041 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     8101 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     8161 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     8221 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     8281 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
     8341 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
     8401 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
     8461 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     8521 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     8581 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
     8641 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     8701 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     8761 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     8821 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     8881 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     8941 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
     9001 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
     9061 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
     9121 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta cttcagaaga actcgtcaag
     9181 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     9241 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     9301 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     9361 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat catcgccgtc
     9421 gggcatacgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     9481 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     9541 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     9601 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     9661 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     9721 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgtag
     9781 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     9841 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     9901 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatatc
     9961 tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt
    10021 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
    10081 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
    10141 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    10201 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    10261 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    10321 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    10381 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    10441 caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    10501 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    10561 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    10621 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    10681 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    10741 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
    10801 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    10861 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    10921 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    10981 caatttcaca caggaaacag ctatgaccat gattacg
//
