LOCUS       V040635                11531 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040635
VERSION     V040635
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        500..845
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /label="CaMV 35S promoter"
     CDS             880..1587
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     terminator      1984..2625
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
                     /label="E9 terminator"
     primer_bind     complement(2633..2649)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     misc_feature    2852..2876
                     /note="right border repeat from nopaline C58 T-DNA"
                     /label="RB T-DNA repeat"
     CDS             4176..4805
                     /codon_start=1
                     /product="stability protein from the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
     CDS             5239..6306
                     /codon_start=1
                     /note=""
                     /product="replication protein from the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /protein_id=""
                     /transl_table=1
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     rep_origin      6372..6566
                     /note="origin of replication for the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     misc_feature    6910..7050
                     /note="basis of mobility region from pBR322"
                     /label="bom"
     rep_origin      complement(7236..7824)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7911..8705)
                     /codon_start=1
                     /gene="aphA-3"
                     /note="confers resistance to kanamycin"
                     /product="aminoglycoside phosphotransferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
                     /label="KanR"
     misc_feature    9130..9154
                     /note="left border repeat from nopaline C58 T-DNA"
                     /label="LB T-DNA repeat"
     polyA_signal    9232..9406
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /label="CaMV poly(A) signal"
     CDS             complement(9446..10471)
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K*"
                     /label="HygR"
     promoter        complement(10539..11216)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /label="CaMV 35S promoter (enhanced)"
     protein_bind    11407..11428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        11443..11473
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11481..11497
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     11505..11521
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagaa tggtgagcaa gggcgaggag
      901 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      961 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1021 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1081 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1141 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1201 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1261 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1321 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1381 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1441 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1501 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1561 accggcggca tggacgagct gtacaaggtc gacgtccaag actataacca tgataaagtt
     1621 gtttcccgag gatcgaaaaa gtatgtggaa gatcttgaaa gtggattcag ctcggatgtc
     1681 gaatcaaaat acaagaaaat ttacgaagat gacatcaacc cttttgcagc attctcgaaa
     1741 aaggaaagag agcaacggat caaagatttg ggaatcagag atcggattac gctaagcagt
     1801 gggcggttcc ttctaggaaa caaatacgca aggacatttg ctttcttcta cacaatagga
     1861 ttgcacgtcc ttgtcttcac ttgtctctac cgtatgtctg cttacagtta tctcagccat
     1921 ggagcagagg agactctaat gacagaagca accacaaacc ttcctcacgg tctttaactg
     1981 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
     2041 cattgcgcac acaccagaat cctactgagt tcgagtatta tggcattggg aaacatgttt
     2101 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     2161 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     2221 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaaatataa
     2281 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     2341 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
     2401 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
     2461 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
     2521 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
     2581 ttaatgcatt ttatgacttg ccaattgatt gacaacatgc atcaagcttg gcactggccg
     2641 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2701 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2761 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
     2821 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     2881 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
     2941 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     3001 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3061 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3121 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3181 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3241 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3301 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3361 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3421 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3481 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3541 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3601 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3661 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3721 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3781 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3841 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3901 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     3961 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     4021 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     4081 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     4141 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     4201 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     4261 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4321 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4381 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4441 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4501 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4561 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4621 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4681 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4741 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4801 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4861 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4921 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     4981 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     5041 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     5101 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     5161 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     5221 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5281 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5341 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5401 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5461 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5521 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5581 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5641 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5701 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5761 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5821 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5881 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5941 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     6001 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     6061 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     6121 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     6181 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6241 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6301 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6361 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6421 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6481 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6541 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6601 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6661 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6721 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6781 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6841 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6901 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6961 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     7021 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     7081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7861 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     7921 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     7981 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     8041 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     8101 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     8161 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     8221 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     8281 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     8341 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     8401 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     8461 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     8521 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     8581 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     8641 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     8701 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     8761 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     8821 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     8881 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     8941 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     9001 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     9061 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     9121 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     9181 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     9241 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     9301 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     9361 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     9421 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     9481 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     9541 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     9601 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     9661 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
     9721 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     9781 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     9841 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     9901 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     9961 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
    10021 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
    10081 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
    10141 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
    10201 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
    10261 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
    10321 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
    10381 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
    10441 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag
    10501 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
    10561 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
    10621 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    10681 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    10741 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    10801 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    10861 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    10921 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt
    10981 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    11041 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    11101 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    11161 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    11221 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    11281 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
    11341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    11401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    11461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    11521 ccatgattac g
//
