LOCUS       62056_4605       10691 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..351
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1530..1782
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2011..2035
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3335..3961
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4393..5463
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5532..5726
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6070..6210
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6396..6984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7074..7865)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8290..8314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8392..8566)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8609..9631)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9699..10376)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10567..10588
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10603..10633
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10641..10657
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10665..10681
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattctgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
       61 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
      121 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
      181 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      241 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
      301 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggacg
      361 agctcggtac ccggggatcc atgggtgcag gtggaagaat gccagtgcct ccatcatcca
      421 aacctatgaa gaggtctcct tactcaaagc caccattcac gctcggtgag ctcaagaagg
      481 ccatccctcc acactgcttc aaacgctcaa tcccccgatc gttcgcctac gtggcgcacg
      541 acctcaccat tgcagcaatc ttctactaca tcgccaccac ttacttccac ctcctcccta
      601 gccctctcaa ctacctcgcc tggccggtct actgggcctg ccagggctgc atcctcactg
      661 gagtatgggt gttggctcac gaatgcggtc accatgcctt cagcgactac cagtggctcg
      721 acgacatgga tggcttcgtc ctccattcgt ccctccttgt tccttacttc tcctggaagc
      781 acagccaccg ccgccaccat tccaacacgg gatcgcttga tcgtgatgag gtgtttgtcc
      841 ccaagcagaa ggccgaaatc gggtggtact ccaagtacct taacaaccca cctggccgtg
      901 tgatcacatt ggccgtcaca ttaacgctcg gttggcctct gtacttggca ttcaacgtct
      961 ccgggagacc atatgaccgg ttcgcatgcc attttgaccc tcacggtccg atttacaatg
     1021 atcgcgagcg tatggagata tacctatccg acgcagggat attcaccgtg tgctacatcc
     1081 tatacagact cgtcctcacg aaaggactcg tttgggtcgt gtccatatac ggagtcccac
     1141 tattgatagt gaatggattc ctagtcctca tcactttctt gcagcacacg catccttctc
     1201 ttccgcacta caagtcctcc gaatgggact ggatgcgagg cgccctctcg accgtggatc
     1261 gagactacgg gttactcaac accgtgttcc acaacatcac cgatacacat gtcgcgcacc
     1321 atctcttctc cacgatgcct cattaccacg cgatggaggc taccaaggcg atcaagccgg
     1381 ttctcgggga gtattaccag ttcgatggga ctccctttgt gaaggccatg tggagggagg
     1441 caaaggagtg catctatgtc gagccggatg aaggcgaccc cagccaaggc gtgttctggt
     1501 acaacaataa gttatgactg caggcatgcg atcgttcaaa catttggcaa taaagtttct
     1561 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     1621 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     1681 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     1741 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcaagcttgg cactggccgt
     1801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1861 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1921 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     1981 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     2041 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     2101 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     2161 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     2221 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     2281 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     2341 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     2401 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     2461 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     2521 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     2581 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     2641 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     2701 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     2761 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     2821 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     2881 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     2941 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     3001 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     3061 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     3121 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     3181 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     3241 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     3301 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     3361 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     3421 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     3481 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     3541 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     3601 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     3661 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     3721 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     3781 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     3841 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     3901 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     3961 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     4021 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     4081 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     4141 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     4201 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     4261 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     4321 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     4381 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     4441 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     4501 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     4561 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     4621 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     4681 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     4741 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     4801 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     4861 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     4921 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     4981 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5041 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     5101 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     5161 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     5221 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     5281 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     5341 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     5401 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     5461 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     5521 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     5581 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     5641 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     5701 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     5761 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     5821 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     5881 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     5941 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6001 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6061 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6121 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6181 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7021 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     7081 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     7141 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     7201 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     7261 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     7321 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     7381 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     7441 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     7501 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     7561 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     7621 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     7681 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     7741 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     7801 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     7861 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     7921 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     7981 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     8041 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     8101 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     8161 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     8221 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     8281 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     8341 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     8401 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     8461 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     8521 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     8581 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     8641 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     8701 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     8761 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     8821 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
     8881 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     8941 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     9001 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     9061 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     9121 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     9181 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     9241 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
     9301 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     9361 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
     9421 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     9481 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     9541 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     9601 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag
     9661 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     9721 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
     9781 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     9841 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     9901 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     9961 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    10021 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    10081 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt
    10141 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    10201 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    10261 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    10321 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    10381 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    10441 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
    10501 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10561 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10621 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10681 ccatgattac g
//
