LOCUS       Exported               13258 bp DNA     circular SYN 06-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          11097..11849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    220..244
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             560..1246
                     /codon_start=1
                     /label=recombinase
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     CDS             1545..2174
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2603..3676
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3742..3936
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4280..4420
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4606..5194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5271..6479)
                     /codon_start=1
                     /label=IS4-like element ISVsa5 family transposase
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL
                     VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ
                     KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYAL
                     GKL"
     CDS             complement(6619..7413)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7838..7862
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7940..8114
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8155..9180)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9247..9923)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10114..10135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10150..10180
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10188..10204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10212..10228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10737..11082
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             11097..11849
                     /gene="TIP1-1"
                     /label=Aquaporin TIP1-1
                     /note="Aquaporin TIP1-1 from Arabidopsis thaliana.
                     Accession#: P25818"
     CDS             11880..12590
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      12610..13251
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      121 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
      181 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
      241 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
      301 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
      361 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
      421 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
      481 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
      541 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
      601 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
      661 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
      721 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
      781 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
      841 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
      901 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
      961 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     1021 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     1081 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     1141 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     1201 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     1261 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     1321 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     1381 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     1441 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     1501 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     1561 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     1621 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     1681 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     1741 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     1801 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     1861 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     1921 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     1981 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     2041 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     2101 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     2161 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     2221 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     2281 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     2341 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     2401 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     2461 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     2521 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     2581 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     2641 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     2701 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     2761 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     2821 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     2881 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     2941 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     3001 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     3061 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     3121 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     3181 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     3241 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     3301 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     3361 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     3421 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     3481 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     3541 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     3601 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     3661 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     3721 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     3781 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     3841 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     3901 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     3961 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     4021 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     4081 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     4141 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4261 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     4321 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4381 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     4441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5041 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5221 gtctgacgct cagtggaacg aaaactcacg ttaagggctg agagatcccc tcataatttc
     5281 cccaaagcgt aaccatgtgt gaataaattt tgagctagta gggttgcagc cacgagtaag
     5341 tcttcccttg ttattgtgta gccagaatgc cgcaaaactt ccatgcctaa gcgaactgtt
     5401 gagagtacgt ttcgatttct gactgtgtta gcctggaagt gcttgtccca accttgtttc
     5461 tgagcatgaa cgcccgcaag ccaacatgtt agttgaagca tcagggcgat tagcagcatg
     5521 atatcaaaac gctctgagct gctcgttcgg ctatggcgta ggcctagtcc gtaggcagga
     5581 cttttcaagt ctcggaaggt ttcttcaatc tgcattcgct tcgaatagat attaacaagt
     5641 tgtttgggtg ttcgaatttc aacaggtaag ttagttgcta gaacccatgg ctcctttgcc
     5701 gacgctgagt agattttagg tgacgggtgg tgacaatgag tccgtgtcga gcgctgattt
     5761 tttcggcctt tagagcgaga tttatacaat agaatttggc atgagattgg attgctttta
     5821 gtcagcctct tatagcctaa agtctttgag tgactagatg acatatcatg taagttgctg
     5881 ataggtttcc agttttccgc tcctaggtct gcatattgta cttttcctct tactcgactt
     5941 aaccagtacc aacccagctt ctcaacggat ttataccatg gcactttaaa gccagcatca
     6001 ctgacaatga gcggtgtggt gttactcggt agaatgctcg caaggtcggc tagaaattgg
     6061 tcatgagctt tctttgaaca ttgctctgaa agcgggaacg ctttctcata aagagtaaca
     6121 gaacgaccgt gtagtgcgac tgaagctcgc aataccataa gtcgtttttg ctcacgaata
     6181 tcagaccagt caacaagtac aatgggcatc gtattgcccg aacagataaa gctagcatgc
     6241 caacggtata cagcgagtcg ctctttgtgg aggtgacgat tacctaacaa tcggtcgatt
     6301 cgtttgatgt tatgttttgt tctcgctttg gttggcaggt tacggccaag ttcggtaaga
     6361 gtgagagttt tacagtcaag taatgcgtgg caagccaacg ttaagctgtt gagtcgtttt
     6421 aagtgtaatt cggggcagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc
     6481 ttgttgtctg attattgatt tttcgcgaaa ccatttgatc atatgacaag atgtgtatcc
     6541 accttaactt aatgattttt accaaaatca ttaggggatt catcagcgtt aagggatttt
     6601 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
     6661 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
     6721 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
     6781 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
     6841 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
     6901 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
     6961 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
     7021 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
     7081 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
     7141 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
     7201 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
     7261 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
     7321 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
     7381 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
     7441 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
     7501 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
     7561 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
     7621 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     7681 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     7741 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     7801 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     7861 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     7921 acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
     7981 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
     8041 caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca
     8101 ttattatgga gaaactcgag cttgtcgatc gacagatccc ggtcggcatc tactctattt
     8161 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     8221 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     8281 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     8341 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
     8401 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     8461 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     8521 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     8581 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     8641 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     8701 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     8761 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     8821 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
     8881 caacgtgaca ccctgtgaac ggcgggagat gcaataggtc aggctctcgc taaactcccc
     8941 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     9001 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     9061 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     9121 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     9181 atctcattgc cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg
     9241 gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     9301 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     9361 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     9421 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     9481 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     9541 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     9601 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     9661 ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     9721 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     9781 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     9841 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     9901 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     9961 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
    10021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10081 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    10141 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    10201 acaatttcac acaggaaaca gctatgacat gattacgaat tcggtcccca gattagcctt
    10261 ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtctc
    10321 atcaagacga tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt
    10381 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
    10441 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg
    10501 caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag
    10561 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata
    10621 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
    10681 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
    10741 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
    10801 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
    10861 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
    10921 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10981 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    11041 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac gagctcatgc
    11101 cgatcagaaa catcgccatt ggccgtccag atgaagccac ccgtcccgat gccttaaagg
    11161 cggcgttggc tgagttcatt tcaactttga tctttgtcgt cgccggttca ggctctggca
    11221 tggctttcaa caagctcact gaaaacggag ccaccactcc ttctggtctc gtagctgctg
    11281 cagtggctca tgcctttgga ctcttcgtcg ctgtctcagt tggtgccaac atctctggtg
    11341 gacacgttaa ccctgccgtc actttcggtg ctttcattgg tggtaacatc actctcctcc
    11401 gtggtatcct ctactggatt gctcagcttc tcggctccgt cgtcgcttgc ctcatcctta
    11461 aattcgccac cggtggcttg gctgtgccgg cttttggtct ctctgctgga gtaggagtgt
    11521 tgaacgcttt cgttttcgag atcgtgatga cattcgggct tgtttacacc gtctacgcta
    11581 cagccattga ccccaaaaac gggagtcttg gaacaattgc tcccatcgca atcggtttca
    11641 ttgttggagc caacatctta gctggaggag ctttctctgg agcctccatg aatcccgccg
    11701 tggctttcgg accagcggtg gtgagctgga catggaccaa ccactgggtc tactgggccg
    11761 gacctctcgt cggcggtgga atcgctggac tcatctacga agttttcttc atcaacacca
    11821 cacacgagca gctcccaacc acagactacg tcgacggtca ccgcgggccc gggatcctga
    11881 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
    11941 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
    12001 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
    12061 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
    12121 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
    12181 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
    12241 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
    12301 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
    12361 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
    12421 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
    12481 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
    12541 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtga tctaggggct
    12601 agaatgcaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat
    12661 cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc attgggaaaa
    12721 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
    12781 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
    12841 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
    12901 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa
    12961 tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc ttattcacta
    13021 ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata caagtatgtc
    13081 ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa tccttgtcag
    13141 attctaatca ttgctttata attatagtta tactcatgga tttgtagttg agtatgaaaa
    13201 tattttttaa tgcattttat gacttgccaa ttgattgaca acatgcatca agcttggc
//
