LOCUS       62056_4635       10384 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        505..850
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             868..894
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             895..1146
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1705..1729
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3029..3655
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4092..5156
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5225..5419
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5763..5903
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6089..6677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6767..7558)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7983..8007
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8085..8259)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8302..9324)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9392..10069)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10260..10281
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10296..10326
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10334..10350
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10358..10374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcaattc ggtccccaga ttagcctttt caatttcaga aagaatgcta acccacagat
       61 ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca ataatctcca
      121 ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
      181 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
      241 gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt
      301 agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact
      361 cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa
      421 aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      481 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      541 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      601 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      661 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      721 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      781 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      841 ggagagaaca cgggggacga gctcatgtac ccatacgatg ttccagatta cgctgacaag
      901 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      961 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1021 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1081 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1141 tacaagggta cccggggatc ctctagagtc gacctgcagg ttcgagtatt atggcattgg
     1201 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     1261 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     1321 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     1381 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
     1441 tctaatgacc gaagttaata tgaggagtaa aacactaagc ttggcactgg ccgtcgtttt
     1501 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     1561 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     1621 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     1681 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     1741 gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     1801 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     1861 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     1921 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     1981 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     2041 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     2101 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     2161 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     2221 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     2281 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     2341 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     2401 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     2461 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     2521 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     2581 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     2641 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     2701 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     2761 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     2821 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     2881 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     2941 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     3001 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     3061 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     3121 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     3181 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     3241 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     3301 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     3361 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     3421 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     3481 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     3541 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     3601 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     3661 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     3721 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     3781 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     3841 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     3901 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     3961 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     4021 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     4081 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     4141 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     4201 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     4261 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     4321 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     4381 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     4441 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     4501 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     4561 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     4621 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     4681 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     4741 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     4801 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     4861 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     4921 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     4981 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     5041 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     5101 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     5161 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     5221 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     5281 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     5341 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     5401 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     5461 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     5521 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     5581 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     5641 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5701 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5761 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     5821 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5881 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6721 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     6781 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     6841 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     6901 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     6961 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     7021 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     7081 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     7141 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     7201 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     7261 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     7321 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     7381 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     7441 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     7501 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     7561 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     7621 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     7681 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     7741 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     7801 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     7861 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     7921 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     7981 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     8041 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     8101 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     8161 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     8221 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
     8281 atccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
     8341 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     8401 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     8461 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     8521 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
     8581 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     8641 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     8701 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     8761 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     8821 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     8881 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     8941 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
     9001 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     9061 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     9121 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     9181 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     9241 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     9301 cgcggtgagt tcaggctttt tcatatctca ttgccccccc ggatctgcga aagctcgaga
     9361 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
     9421 ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     9481 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     9541 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     9601 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     9661 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     9721 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     9781 cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca cttgctttga
     9841 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     9901 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     9961 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
    10021 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
    10081 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    10141 gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10261 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10321 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    10381 tacg
//
