LOCUS       62056_4610       10469 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        505..850
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             868..894
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             895..1146
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1790..1814
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3114..3740
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4177..5241
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5310..5504
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5848..5988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6174..6762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6852..7643)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8068..8092
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8170..8344)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8387..9409)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9477..10154)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10345..10366
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10381..10411
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10419..10435
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10443..10459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcaattc ggtccccaga ttagcctttt caatttcaga aagaatgcta acccacagat
       61 ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca ataatctcca
      121 ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
      181 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
      241 gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt
      301 agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact
      361 cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa
      421 aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      481 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      541 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      601 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      661 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      721 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      781 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      841 ggagagaaca cgggggacga gctcatgtac ccatacgatg ttccagatta cgctgacaag
      901 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      961 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1021 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1081 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1141 tacaagggta cccggggatc ctctagagtc gacctgcaga gctttcgttc gtatcatcgg
     1201 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta
     1261 ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg
     1321 taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga
     1381 gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt
     1441 tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg
     1501 agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac
     1561 taagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1621 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1681 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga
     1741 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
     1801 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc
     1861 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     1921 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     1981 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     2041 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     2101 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     2161 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     2221 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     2281 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     2341 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     2401 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     2461 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     2521 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     2581 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     2641 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     2701 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     2761 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     2821 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     2881 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     2941 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     3001 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     3061 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     3121 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     3181 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3241 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     3301 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     3361 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     3421 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     3481 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     3541 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     3601 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     3661 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     3721 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     3781 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     3841 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     3901 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     3961 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4021 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     4081 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     4141 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     4201 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     4261 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     4321 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     4381 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     4441 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     4501 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     4561 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     4621 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     4681 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     4741 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     4801 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     4861 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     4921 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     4981 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     5041 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     5101 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     5161 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     5221 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     5281 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     5341 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     5401 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     5461 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     5521 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     5581 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     5641 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     5701 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5761 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5821 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     5881 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5941 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     6001 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6061 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6601 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     6841 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     6901 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     6961 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     7021 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     7081 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     7141 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     7201 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     7261 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     7321 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     7381 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     7441 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     7501 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     7561 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     7621 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     7681 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     7741 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     7801 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     7861 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     7921 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     7981 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     8041 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     8101 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     8161 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     8221 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
     8281 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
     8341 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg
     8401 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     8461 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     8521 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     8581 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
     8641 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     8701 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     8761 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     8821 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     8881 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     8941 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     9001 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
     9061 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     9121 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
     9181 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     9241 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     9301 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     9361 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
     9421 cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
     9481 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
     9541 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     9601 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     9661 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     9721 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     9781 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     9841 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     9901 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     9961 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    10021 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    10081 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    10141 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    10201 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
    10261 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    10321 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10381 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10441 cacaggaaac agctatgacc atgattacg
//
