LOCUS       V039998                11541 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039998
VERSION     V039998
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        500..845
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /label="CaMV 35S promoter"
     CDS             1256..1963
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     terminator      1994..2635
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
                     /label="E9 terminator"
     primer_bind     complement(2643..2659)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     misc_feature    2862..2886
                     /note="right border repeat from nopaline C58 T-DNA"
                     /label="RB T-DNA repeat"
     CDS             4186..4815
                     /codon_start=1
                     /product="stability protein from the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
     CDS             5249..6316
                     /codon_start=1
                     /note=""
                     /product="replication protein from the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /protein_id=""
                     /transl_table=1
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     rep_origin      6382..6576
                     /note="origin of replication for the&nbsp;Pseudomonas
                     plasmid pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     misc_feature    6920..7060
                     /note="basis of mobility region from pBR322"
                     /label="bom"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7921..8715)
                     /codon_start=1
                     /gene="aphA-3"
                     /note="confers resistance to kanamycin"
                     /product="aminoglycoside phosphotransferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
                     /label="KanR"
     misc_feature    9140..9164
                     /note="left border repeat from nopaline C58 T-DNA"
                     /label="LB T-DNA repeat"
     polyA_signal    9242..9416
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /label="CaMV poly(A) signal"
     CDS             complement(9456..10481)
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K*"
                     /label="HygR"
     promoter        complement(10549..11226)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /label="CaMV 35S promoter (enhanced)"
     protein_bind    11417..11438
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        11453..11483
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11491..11507
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     11515..11531
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctca tgagtctgtt ccatgtgcta gggtttggtg tcaagattgg
      901 gcatctcttc tggatgctat gctgctggtt tgtttcttgg ttcgttgata atgggatcga
      961 ggacaagtct ggtcttttag ttggctctgt cggtgatctt gagaagacta agatgactac
     1021 gttgaagaag aagaacaaga tgtggttctg gaataagatc tctagcagcg gactcaagat
     1081 cccgagtttc tcttatcagt ttcttggctc tgttaaattc aacaaggcgt ggtggaggaa
     1141 gcttgtggtg gtttgggttg tcttctgggt cttggtctct atttggacgt tttggtactt
     1201 tagctcgtct agagggcccg gcgcgccact agtgtcgacc agatctcgta cgcgtatggt
     1261 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     1321 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1381 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1441 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     1501 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1561 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1621 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1681 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1741 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1801 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1861 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1921 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagcaactcg agcatgacga
     1981 gctgtagctg cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat
     2041 gcatcagttt cattgcgcac acaccagaat cctactgagt tcgagtatta tggcattggg
     2101 aaacatgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt
     2161 ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct
     2221 tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt
     2281 gaaaatataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct
     2341 ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc
     2401 actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta
     2461 tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg
     2521 tcagattcta atcattgctt tataattata gttatactca tggatttgta gttgagtatg
     2581 aaaatatttt ttaatgcatt ttatgacttg ccaattgatt gacaacatgc atcaagcttg
     2641 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2701 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2761 cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat
     2821 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     2881 gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag
     2941 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     3001 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     3061 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     3121 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     3181 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     3241 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     3301 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     3361 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     3421 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     3481 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     3541 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     3601 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     3661 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     3721 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     3781 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     3841 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     3901 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     3961 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4021 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4081 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4141 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4201 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4261 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4321 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4381 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     4441 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     4501 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     4561 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     4621 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     4681 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     4741 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     4801 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     4861 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     4921 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     4981 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5041 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5101 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5161 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     5221 acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg
     5281 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     5341 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     5401 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     5461 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     5521 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     5581 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     5641 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     5701 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     5761 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     5821 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     5881 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     5941 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6001 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6061 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     6121 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     6181 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6241 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6301 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     6361 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     6421 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     6481 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     6541 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     6601 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     6661 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     6721 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     6781 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     6841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6901 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     6961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     7921 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     7981 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     8041 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     8101 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     8161 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     8221 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     8281 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     8341 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     8401 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     8461 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     8521 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     8581 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     8641 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     8701 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     8761 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     8821 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     8881 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     8941 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     9001 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     9061 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9121 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     9181 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     9241 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     9301 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     9361 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     9421 agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg
     9481 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     9541 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     9601 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     9661 agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc
     9721 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     9781 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     9841 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     9901 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     9961 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
    10021 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
    10081 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc
    10141 ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
    10201 cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga
    10261 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
    10321 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
    10381 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
    10441 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga
    10501 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
    10561 ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt
    10621 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
    10681 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
    10741 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
    10801 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
    10861 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
    10921 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat
    10981 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
    11041 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
    11101 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
    11161 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
    11221 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    11281 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
    11341 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    11401 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11461 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11521 acagctatga ccatgattac g
//
