LOCUS       62056_4600       11195 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             922..1638
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1648..2289
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2516..2540
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3840..4466
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4903..5967
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6036..6230
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6574..6714
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6900..7488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7578..8369)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8794..8818
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8896..9070)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9113..10135)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10203..10880)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11071..11092
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11107..11137
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11145..11161
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11169..11185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagtg
      901 tcgaccagat ctcgtacgcg tatggtgagc aagggcgagg agctgttcac cggggtggtg
      961 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1021 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1081 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1141 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1201 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1261 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1321 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1381 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1441 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1501 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1561 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1621 atggacgagc tgtacaagtg actgcagagc tttcgttcgt atcatcggtt tcgacaacgt
     1681 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagttcgagt
     1741 attatggcat tgggaaacat gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1801 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1861 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1921 tgtgtgttga atttgaaaat ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1981 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     2041 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     2101 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     2161 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     2221 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
     2281 atgcatcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2341 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2401 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     2461 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     2521 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
     2581 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2641 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     2701 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     2761 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     2821 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     2881 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     2941 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     3001 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     3061 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     3121 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     3181 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     3241 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     3301 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     3361 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     3421 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     3481 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     3541 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     3601 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     3661 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     3721 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     3781 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     3841 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     3901 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     3961 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     4021 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     4081 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     4141 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     4201 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     4261 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     4321 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     4381 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     4441 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     4501 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     4561 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     4621 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     4681 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     4741 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     4801 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     4861 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa accatccggc
     4921 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     4981 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     5041 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     5101 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     5161 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     5221 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     5281 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     5341 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     5401 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     5461 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     5521 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     5581 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     5641 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     5701 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     5761 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     5821 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     5881 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     5941 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     6001 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     6061 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     6121 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     6181 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     6241 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     6301 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     6361 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     6421 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6481 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6541 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     6601 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6661 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     6721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7321 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7561 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     7621 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     7681 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     7741 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     7801 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     7861 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
     7921 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     7981 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     8041 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     8101 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
     8161 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     8221 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     8281 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     8341 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     8401 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     8461 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     8521 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     8581 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     8641 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     8701 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     8761 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     8821 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     8881 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     8941 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     9001 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     9061 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg
     9121 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     9181 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     9241 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     9301 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
     9361 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     9421 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     9481 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     9541 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     9601 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     9661 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     9721 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     9781 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     9841 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
     9901 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     9961 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
    10021 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
    10081 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
    10141 attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
    10201 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
    10261 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
    10321 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
    10381 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
    10441 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
    10501 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
    10561 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    10621 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
    10681 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
    10741 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
    10801 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
    10861 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
    10921 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa
    10981 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11041 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11101 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    11161 atttcacaca ggaaacagct atgaccatga ttacg
//
