LOCUS       62056_4595       11540 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             880..1596
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1993..2634
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2861..2885
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4185..4811
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5248..6312
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6381..6575
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6919..7059
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7245..7833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7923..8714)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9139..9163
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9241..9415)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9458..10480)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10548..11225)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11416..11437
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11452..11482
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11490..11506
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11514..11530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagaa tggtgagcaa gggcgaggag
      901 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      961 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1021 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1081 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1141 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1201 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1261 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1321 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1381 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1441 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1501 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1561 gccgggatca ctctcggcat ggacgagctg tacaaggtcg acgtccaaga ctataaccat
     1621 gataaagttg tttcccgagg atcgaaaaag tatgtggaag atcttgaaag tggattcagc
     1681 tcggatgtcg aatcaaaata caagaaaatt tacgaagatg acatcaaccc ttttgcagca
     1741 ttctcgaaaa aggaaagaga gcaacggatc aaagatttgg gaatcagaga tcggattacg
     1801 ctaagcagtg ggcggttcct tctaggaaac aaatacgcaa ggacatttgc tttcttctac
     1861 acaataggat tgcacgtcct tgtcttcact tgtctctacc gtatgtctgc ttacagttat
     1921 ctcagccatg gagcagagga gactctaatg acagaagcaa ccacaaacct tcctcacggt
     1981 ctttaactgc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg
     2041 catcagtttc attgcgcaca caccagaatc ctactgagtt cgagtattat ggcattggga
     2101 aacatgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     2161 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     2221 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     2281 aaaatataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
     2341 taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta tgcttattca
     2401 ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga atacaagtat
     2461 gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca gaatccttgt
     2521 cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag ttgagtatga
     2581 aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca tcaagcttgg
     2641 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2701 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2761 gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc
     2821 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     2881 taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg
     2941 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3001 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     3061 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     3121 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     3181 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     3241 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     3301 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     3361 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     3421 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     3481 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     3541 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     3601 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     3661 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     3721 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     3781 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     3841 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     3901 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     3961 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     4021 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     4081 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     4141 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     4201 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     4261 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     4321 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     4381 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     4441 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     4501 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     4561 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     4621 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     4681 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     4741 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     4801 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     4861 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     4921 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     4981 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     5041 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     5101 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     5161 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     5221 cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc
     5281 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     5341 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     5401 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     5461 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     5521 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     5581 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     5641 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     5701 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     5761 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     5821 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     5881 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     5941 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     6001 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     6061 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     6121 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     6181 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     6241 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     6301 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     6361 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     6421 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     6481 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     6541 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     6601 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     6661 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     6721 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     6781 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6841 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6901 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     6961 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     7021 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     7081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7681 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
     7921 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     7981 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     8041 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     8101 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     8161 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     8221 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     8281 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     8341 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     8401 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     8461 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     8521 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     8581 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     8641 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     8701 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     8761 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     8821 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     8881 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     8941 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     9001 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     9061 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     9121 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     9181 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     9241 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     9301 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     9361 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     9421 gcttgtcgat cgacagatcc ggtcggcatc tactctattt ctttgccctc ggacgagtgc
     9481 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     9541 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     9601 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     9661 gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg
     9721 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     9781 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     9841 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     9901 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     9961 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
    10021 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
    10081 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg
    10141 gttgtagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
    10201 ggcgggagat gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa
    10261 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
    10321 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
    10381 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
    10441 acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccgggat
    10501 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
    10561 tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg
    10621 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
    10681 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    10741 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    10801 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    10861 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    10921 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc
    10981 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    11041 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    11101 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    11161 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    11221 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    11281 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
    11341 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11401 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11461 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11521 cagctatgac catgattacg
//
