LOCUS       62056_4590       11550 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1256..1972
                     /codon_start=1
                     /label=CFP
                     /note="cyan variant of GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      2003..2644
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2871..2895
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4195..4821
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5258..6322
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6391..6585
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6929..7069
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7255..7843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7933..8724)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9149..9173
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9251..9425)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9468..10490)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10558..11235)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11426..11447
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11462..11492
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11500..11516
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11524..11540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctca tgagtctgtt ccatgtgcta gggtttggtg tcaagattgg
      901 gcatctcttc tggatgctat gctgctggtt tgtttcttgg ttcgttgata atgggatcga
      961 ggacaagtct ggtcttttag ttggctctgt cggtgatctt gagaagacta agatgactac
     1021 gttgaagaag aagaacaaga tgtggttctg gaataagatc tctagcagcg gactcaagat
     1081 cccgagtttc tcttatcagt ttcttggctc tgttaaattc aacaaggcgt ggtggaggaa
     1141 gcttgtggtg gtttgggttg tcttctgggt cttggtctct atttggacgt tttggtactt
     1201 tagctcgtct agagggcccg gcgcgccact agtgtcgacc agatctcgta cgcgtatggt
     1261 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1321 cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1381 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1441 gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1501 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa
     1561 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1621 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1681 ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     1741 caaggcccac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1801 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1861 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1921 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agcaactcga
     1981 gcatgacgag ctgtagctgc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc
     2041 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt cgagtattat
     2101 ggcattggga aacatgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     2161 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     2221 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     2281 gttgaatttg aaaatataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     2341 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
     2401 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
     2461 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
     2521 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
     2581 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca
     2641 tcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2701 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2761 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
     2821 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
     2881 tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg
     2941 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     3001 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     3061 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     3121 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     3181 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     3241 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     3301 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     3361 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     3421 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     3481 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     3541 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     3601 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     3661 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     3721 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     3781 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     3841 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     3901 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     3961 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat
     4021 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     4081 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     4141 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     4201 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     4261 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     4321 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     4381 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     4441 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     4501 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     4561 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     4621 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     4681 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     4741 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     4801 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     4861 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     4921 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     4981 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     5041 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     5101 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     5161 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat
     5221 ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt
     5281 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     5341 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     5401 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     5461 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     5521 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     5581 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     5641 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     5701 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     5761 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     5821 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     5881 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     5941 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     6001 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     6061 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     6121 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     6181 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     6241 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     6301 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     6361 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     6421 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     6481 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     6541 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     6601 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     6661 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     6721 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     6781 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6841 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6901 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     6961 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     7021 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     7081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7141 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7201 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7261 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7321 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7381 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7441 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7501 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7561 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7621 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7681 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7741 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7801 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7861 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
     7921 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
     7981 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
     8041 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
     8101 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
     8161 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
     8221 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
     8281 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
     8341 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
     8401 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
     8461 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
     8521 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
     8581 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
     8641 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
     8701 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
     8761 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     8821 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
     8881 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
     8941 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
     9001 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     9061 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     9121 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     9181 cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat
     9241 taattcgggg gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     9301 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     9361 agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg
     9421 agaaactcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt ctttgccctc
     9481 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     9541 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     9601 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     9661 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct
     9721 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     9781 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     9841 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     9901 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     9961 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
    10021 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
    10081 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatagcc
    10141 tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
    10201 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc aatgtcaagc
    10261 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
    10321 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
    10381 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
    10441 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
    10501 cccccgggat ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca
    10561 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag
    10621 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
    10681 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    10741 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    10801 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    10861 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    10921 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
    10981 ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    11041 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    11101 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    11161 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    11221 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    11281 tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc
    11341 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    11401 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11461 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11521 acacaggaaa cagctatgac catgattacg
//
