LOCUS       62056_4585       11270 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             985..1692
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1723..2364
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2591..2615
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3915..4541
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4978..6042
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6111..6305
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6649..6789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6975..7563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7653..8444)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8869..8893
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8971..9145)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9188..10210)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10278..10955)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11146..11167
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11182..11212
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11220..11236
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11244..11260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctct ctagagggcc cggcgcgcca ctagtatgaa ggtacaggag
      901 ggtttgttcg tggtggctgt tttctacctt gcttatacgc agctagtcaa ggggcaacct
      961 gtgtcgacca gatctcgtac gcgtatggtg agcaagggcg aggaggataa catggccatc
     1021 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1081 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1141 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1201 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1261 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1321 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1381 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1441 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1501 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1561 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1621 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1681 gagctgtaca agcaactcga gcatgacgag ctgtagctgc agagctttcg ttcgtatcat
     1741 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
     1801 ctactgagtt cgagtattat ggcattggga aacatgtttt tcttgtacca tttgttgtgc
     1861 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
     1921 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
     1981 ttttctctta tttgttgtgt gttgaatttg aaaatataag agatatgcaa acattttgtt
     2041 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
     2101 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
     2161 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
     2221 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
     2281 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
     2341 caattgattg acaacatgca tcaagcttgg cactggccgt cgttttacaa cgtcgtgact
     2401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2521 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     2581 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     2641 ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2701 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2761 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     2821 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2881 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2941 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     3001 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     3061 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     3121 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     3181 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     3241 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     3301 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     3361 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3421 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3481 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3541 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3601 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3661 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     3721 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3781 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3841 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     3901 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3961 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     4021 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     4081 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     4141 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     4201 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     4261 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     4321 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     4381 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4441 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4501 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4561 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4621 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4681 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4741 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4801 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4861 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4921 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     4981 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     5041 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     5101 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     5161 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     5221 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     5281 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     5341 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     5401 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     5461 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     5521 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     5581 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     5641 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     5701 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     5761 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     5821 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     5881 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     5941 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     6001 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     6061 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     6121 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     6181 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     6241 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     6301 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     6361 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     6421 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     6481 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6541 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6601 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     6661 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     6721 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6781 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7381 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7621 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
     7681 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
     7741 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
     7801 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
     7861 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
     7921 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
     7981 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
     8041 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
     8101 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
     8161 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
     8221 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
     8281 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
     8341 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
     8401 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
     8461 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
     8521 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
     8581 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
     8641 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     8701 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     8761 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     8821 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     8881 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     8941 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     9001 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     9061 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     9121 ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc
     9181 tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     9241 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     9301 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     9361 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     9421 cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     9481 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     9541 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     9601 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     9661 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     9721 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     9781 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     9841 cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag
     9901 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     9961 taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    10021 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    10081 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    10141 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    10201 gctttttcat atctcattgc cccccgggat ctgcgaaagc tcgagagaga tagatttgta
    10261 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
    10321 gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
    10381 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
    10441 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
    10501 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
    10561 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
    10621 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
    10681 gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
    10741 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
    10801 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
    10861 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
    10921 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
    10981 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct
    11041 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11101 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11161 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11221 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
//
