LOCUS       pCAMBIA-2301       11633 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Binary vector pCAMBIA-2301, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Hajdukiewicz P, Svab Z, Maliga P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors for
            plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
  PUBMED    7919218
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     A comprehensive set of modular vectors for advanced manipulations 
            and efficient transformation of plants
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11633)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / 
            GPO Box 3200, Canberra, ACT 2601, Australia
REFERENCE   4  (bases 1 to 11633)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11633)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 11633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol.
            Biol."; date: "1994"; volume: "25"; issue: "6"; pages: "989-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box
            3200, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     exon            2..16
                     /note="synthetic first gus exon (modular)"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             201..2024
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2031..2048
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2083..2335
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3682..4308
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4746..5810
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5879..6073
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6417..6557
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6743..7331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7421..8212)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8637..8661
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8739..8913)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8973..9761)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(9830..10506)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10697..10718
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10733..10763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10771..10787
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10795..10811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10820..10876
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10880..10896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11273..11618
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     2461 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     2521 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     2581 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     2641 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     2701 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     2761 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     2821 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     2881 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     2941 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     3001 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     3061 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     3121 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     3181 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     3241 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     3301 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     3361 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     3421 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     3481 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     3541 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     3601 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     3661 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     3721 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     3781 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     3841 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     3901 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     3961 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     4021 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     4081 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     4141 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     4201 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     4261 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     4321 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     4381 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     4441 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     4501 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     4561 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     4621 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     4681 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     4741 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     4801 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     4861 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     4921 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     4981 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     5041 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     5101 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     5161 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     5221 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     5281 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     5341 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     5401 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     5461 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     5521 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     5581 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     5641 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     5701 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     5761 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     5821 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     5881 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     5941 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     6001 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     6061 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     6121 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     6181 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     6241 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     6301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     6421 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     6481 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6541 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7381 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
     7441 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     7501 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     7561 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     7621 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     7681 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     7741 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     7801 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     7861 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     7921 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     7981 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     8041 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     8101 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     8161 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     8221 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     8281 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     8341 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
     8401 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     8461 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     8521 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     8581 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     8641 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     8701 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     8761 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     8821 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta
     8881 tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg actctagcta
     8941 gaggatcgat ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     9001 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     9061 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     9121 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     9181 ttcggcaagc aggcatcgcc atgtgtcacg acgagatcct cgccgtcggg catgcgcgcc
     9241 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     9301 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     9361 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     9421 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     9481 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     9541 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctggagttc attcagggca
     9601 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccgaaacacg
     9661 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     9721 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tccccatggt cgatcgacag
     9781 atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc
     9841 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
     9901 gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg
     9961 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
    10021 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
    10081 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
    10141 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
    10201 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat
    10261 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
    10321 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
    10381 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
    10441 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
    10501 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    10561 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
    10621 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10681 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10741 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10801 acagctatga catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct
    10861 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    10921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    10981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
    11041 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa
    11101 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
    11161 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
    11221 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
    11281 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
    11341 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
    11401 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    11461 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    11521 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    11581 cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt gac
//
