LOCUS       Exported                8744 bp DNA     circular SYN 10-SEP-2025
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            kanamycin-resistance genes.
ACCESSION   AF234315
VERSION     .
KEYWORDS    pCAMBIA2300
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Cambia
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8744)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The GenBank record was corrected by inserting a G at position 2281.
FEATURES             Location/Qualifiers
     source          1..8744
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(641..8744,1..640)
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        167..197
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             241..474
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="lacZ-alpha"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    255..311
                     /label=MCS
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    534..558
                     /label=RB T-DNA repeat
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1859..2488
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=stability protein from Pseudomonas plasmid pVS1
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2917..3990
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /label=replication protein from Pseudomonas plasmid
                     pVS1
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4056..4250
                     /label=pVS1 oriV
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4594..4734
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4920..5508)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5595..6389)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6814..6838
                     /label=LB T-DNA repeat
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6916..7090
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7147..7944)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII)
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8007..8684)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
       61 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
      301 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc
      481 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
      541 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
      601 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
      661 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
      721 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
      781 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
      841 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
      901 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
      961 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     1021 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     1081 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     1141 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     1201 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     1261 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     1321 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     1381 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     1441 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     1501 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     1561 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     1621 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     1681 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     1741 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     1801 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     1861 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     1921 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     1981 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     2041 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     2101 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     2161 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     2221 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     2281 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     2341 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     2401 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     2461 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     2521 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     2581 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     2641 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     2701 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     2761 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     2821 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
     2881 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     2941 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     3001 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     3061 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     3121 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     3181 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     3241 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     3301 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     3361 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     3421 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     3481 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     3541 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     3601 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     3661 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     3721 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     3781 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     3841 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     3901 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     3961 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     4021 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     4081 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     4141 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     4201 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     4261 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     4321 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     4381 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     4441 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4501 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4561 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     4621 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4681 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     4741 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4801 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5341 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5581 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     5641 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     5701 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     5761 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     5821 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     5881 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
     5941 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     6001 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     6061 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     6121 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
     6181 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     6241 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     6301 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     6361 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     6421 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     6481 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     6541 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     6601 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6661 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6721 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6781 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6841 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6901 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     6961 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     7021 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     7081 tatggagaaa ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag
     7141 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     7201 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     7261 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     7321 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     7381 tgtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     7441 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     7501 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     7561 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     7621 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     7681 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     7741 tagccgcgct gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     7801 aagaaccggg cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt
     7861 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     7921 caatccatct tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat
     7981 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
     8041 tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     8101 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     8161 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
     8221 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
     8281 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
     8341 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
     8401 agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg
     8461 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     8521 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     8581 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     8641 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     8701 ctgagactgt atctttgata ttcttggagt agacgagagt gtcg
//
