LOCUS       40924_8796        8894 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA-2200, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Hajdukiewicz P, Svab Z, Maliga P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors for
            plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
  PUBMED    7919218
REFERENCE   2  (bases 1 to 8894)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     A comprehensive set of modular vectors for advanced manipulations 
            and efficient transformation of plants
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8894)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / 
            GPO Box 3200, Canberra, ACT 2601, Australia
REFERENCE   4  (bases 1 to 8894)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1994"; volume: "25"; issue: "6"; pages: "989-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 
            3200, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2282..3346
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5141..5797)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    6325..6349
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6427..6601)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6661..7449)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7518..8195)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8386..8407
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8422..8452
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8460..8476
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8484..8500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8510..8566
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8570..8586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8789..8813
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4921 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
     4981 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     5041 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
     5101 tagctagagg atcgcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     5161 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     5221 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     5281 ttgtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     5341 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     5401 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     5461 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     5521 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     5581 actccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     5641 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     5701 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     5761 tatcaacggt ggtatatcca gtgatttttt tctccatgat gtttaacttt gttttagggc
     5821 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     5881 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa catgtcataa
     5941 caagaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     6001 gaccagttgc gtgacggcag ttacgctact tgcattacag cttacgaacc gaacgaggct
     6061 tatgtccact gggttcgtgc ccgaattgat cacaggcagc aacgctctgt catcgttaca
     6121 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     6181 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     6241 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     6301 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     6361 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     6421 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     6481 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     6541 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     6601 actcgagctt gtcgatcgac tctagctaga ggatcgatcc gaaccccaga gtcccgctca
     6661 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     6721 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     6781 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     6841 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gtgtcacgac
     6901 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     6961 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     7021 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     7081 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     7141 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     7201 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     7261 tgcctcgtcc tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     7321 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     7381 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     7441 ttgttcaatc cccatggtcg atcgacagat ctgcgaaagc tcgagagaga tagatttgta
     7501 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     7561 gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7621 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7681 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7741 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7801 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     7861 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     7921 gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
     7981 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     8041 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     8101 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     8161 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     8221 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct
     8281 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8461 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     8521 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
     8581 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8641 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8701 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     8761 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     8821 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg
     8881 tccatttgta tgtg
//
