LOCUS       Exported               10549 bp DNA     circular SYN 10-SEP-2025
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and kanamycin-resistance and GFP genes.
ACCESSION   AF234298
VERSION     .
KEYWORDS    pCAMBIA1302
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Cambia
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10549)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The GenBank record was corrected by inserting a G at position 3443.
FEATURES             Location/Qualifiers
     source          1..10549
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(1090..10549,1..1089)
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..219
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    227..243
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     251..267
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             263..495
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="lacZ-alpha"
                     /translation="MT*LRIRARYPGIL*SRPAGMQAWHWPSFYNVVTGKTLALPNLIA
                     LQHIPLSPAGVIAKRPAPIALPNSCAA*MANA"
     misc_feature    276..332
                     /label=MCS
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(336..352)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        729..1074
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1106..1816
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="mgfp5"
                     /note="suitable for expression in plants due to removal of
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1823..1840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis affinity tag
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      1872..2124
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2146..2170
                     /label=RB T-DNA repeat
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3471..4100
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=stability protein from Pseudomonas plasmid pVS1
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4529..5602
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /label=replication protein from Pseudomonas plasmid
                     pVS1
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5668..5862
                     /label=pVS1 oriV
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6206..6346
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6532..7120)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7207..8001)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8426..8450
                     /label=LB T-DNA repeat
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8528..8702
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8743..9768)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=hygromycin B phosphotransferase
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9835..10511)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
       61 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      121 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      181 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      241 caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcggt acccggggat
      301 cctctagagt cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
      361 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      421 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      481 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
      541 agcttcatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
      601 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
      661 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
      721 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
      781 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
      841 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
      901 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
      961 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1021 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1081 actcttgacc atggtagatc tgactagtaa aggagaagaa cttttcactg gagttgtccc
     1141 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1201 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1261 acctgttccg tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcaag
     1321 atacccagat catatgaagc ggcacgactt cttcaagagc gccatgcctg agggatacgt
     1381 gcaggagagg accatcttct tcaaggacga cgggaactac aagacacgtg ctgaagtcaa
     1441 gtttgaggga gacaccctcg tcaacaggat cgagcttaag ggaatcgatt tcaaggagga
     1501 cggaaacatc ctcggccaca agttggaata caactacaac tcccacaacg tatacatcat
     1561 ggccgacaag caaaagaacg gcatcaaagc caacttcaag acccgccaca acatcgaaga
     1621 cggcggcgtg caactcgctg atcattatca acaaaatact ccaattggcg atggccctgt
     1681 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1741 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1801 ggatgaacta tacaaagcta gccaccacca ccaccaccac gtgtgaattg gtgaccagct
     1861 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1921 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1981 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2041 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2101 cggtgtcatc tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg
     2161 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     2221 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     2281 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     2341 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     2401 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     2461 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     2521 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     2581 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     2641 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     2701 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     2761 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     2821 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     2881 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     2941 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     3001 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     3061 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     3121 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     3181 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     3241 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     3301 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     3361 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     3421 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     3481 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     3541 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     3601 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     3661 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     3721 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     3781 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     3841 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     3901 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     3961 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     4021 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     4081 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     4141 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     4201 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     4261 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     4321 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     4381 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     4441 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     4501 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     4561 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     4621 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     4681 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     4741 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     4801 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     4861 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     4921 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     4981 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     5041 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     5101 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     5161 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     5221 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     5281 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     5341 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     5401 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     5461 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     5521 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     5581 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     5641 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     5701 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     5761 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     5821 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     5881 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     5941 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     6001 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     6061 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6181 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6241 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6301 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6361 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6721 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6961 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     7201 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
     7261 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
     7321 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
     7381 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
     7441 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
     7501 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
     7561 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
     7621 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
     7681 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
     7741 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
     7801 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
     7861 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
     7921 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
     7981 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
     8041 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
     8101 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
     8161 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
     8221 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     8281 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     8341 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     8401 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     8461 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     8521 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     8581 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     8641 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     8701 aactcgagct tgtcgatcga cagatcccgg tcggcatcta ctctatttct ttgccctcgg
     8761 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     8821 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     8881 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     8941 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
     9001 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     9061 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     9121 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     9181 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     9241 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     9301 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     9361 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc
     9421 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     9481 ctgtgaacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac
     9541 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     9601 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     9661 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     9721 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
     9781 cccggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
     9841 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     9901 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     9961 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
    10021 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
    10081 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
    10141 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
    10201 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    10261 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    10321 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    10381 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    10441 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    10501 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatatt
//
