LOCUS       Exported                8958 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Binary vector pCAMBIA-1300, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Hajdukiewicz P, Svab Z, Maliga P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors for
            plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
  PUBMED    7919218
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     A comprehensive set of modular vectors for advanced manipulations 
            and efficient transformation of plants
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8958)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / 
            GPO Box 3200, Canberra, ACT 2601, Australia
REFERENCE   4  (bases 1 to 8958)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8958)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 8958)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol.
            Biol."; date: "1994"; volume: "25"; issue: "6"; pages: "989-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box
            3200, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..76)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    279..303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1604..2230
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2668..3732
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3801..3995
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4339..4479
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4665..5253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5343..6134)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6559..6583
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6661..6835)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6879..7901)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7968..8644)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8835..8856
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8871..8901
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8909..8925
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8933..8949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8958
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
       61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
      241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
      301 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
      361 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      421 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
      481 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
      541 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
      601 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
      661 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
      721 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
      781 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
      841 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
      901 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
      961 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     1021 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     1081 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     1141 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     1201 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     1261 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     1321 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     1381 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     1441 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     1501 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     1561 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     1621 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     1681 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     1741 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     1801 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     1861 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     1921 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     1981 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     2041 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     2101 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     2161 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     2221 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     2281 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     2341 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     2401 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     2461 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     2521 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     2581 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     2641 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     2701 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     2761 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     2821 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     2881 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     2941 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     3001 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     3061 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     3121 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     3181 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     3241 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     3301 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     3361 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     3421 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     3481 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     3541 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     3601 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     3661 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     3721 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     3781 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     3841 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     3901 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     3961 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     4021 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     4081 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     4141 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     4201 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4261 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4321 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     4381 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     4441 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     4501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5101 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
     5341 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     5401 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     5461 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     5521 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     5581 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     5641 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     5701 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     5761 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     5821 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     5881 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     5941 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     6001 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     6061 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     6121 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     6181 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     6241 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     6301 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     6361 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     6421 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     6481 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     6541 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     6601 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     6661 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     6721 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     6781 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     6841 gcttgtcgat cgacagatcc cggtcggcat ctactctatt tctttgccct cggacgagtg
     6901 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     6961 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     7021 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     7081 agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc
     7141 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     7201 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     7261 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     7321 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     7381 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     7441 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     7501 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc
     7561 ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa
     7621 cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga
     7681 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     7741 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     7801 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     7861 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat
     7921 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     7981 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     8041 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     8101 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     8161 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     8221 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     8281 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     8341 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     8401 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     8461 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     8521 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     8581 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     8641 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     8701 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
     8761 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8821 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8881 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8941 agctatgaca tgattacg
//
