LOCUS       40924_8651       10110 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant transformation vector pCAM-PROM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.
  TITLE     Evaluation of four phloem-specific promoters in vegetative tissues 
            of transgenic citrus plants
  JOURNAL   Tree Physiol. 32 (1), 83-93 (2012)
  PUBMED    22228816
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Dutt M, Ananthakrishnan G, Jaromin M, Brlansky R, Grosser J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2012) Citrus Research and Education Center, 
            University of Florida, 700 Experiment Station Road, Lake Alfred, FL 
            33850, USA
REFERENCE   3  (bases 1 to 10110)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tree 
            Physiol."; date: "2012"; volume: "32"; issue: "1"; pages: "83-93"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-FEB-2012) Citrus Research and Education Center, University of 
            Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             41..1849
                     /codon_start=1
                     /gene="gus"
                     /product="GUS"
                     /label=gus
                     /protein_id="AFM76958.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            41..1849
                     /gene="gus"
                     /label=gus
     regulatory      1864..2154
                     /label=35S - 3'
                     /note="35S - 3'"
                     /regulatory_class="terminator"
     polyA_signal    1908..2082
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2284..2467
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2485..3273
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3333..3507
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(3585..3609)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4034..4825
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4915..5503
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(5689..5829)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6173..6367)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6436..7500)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7937..8563)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(9863..9887)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10090..10106
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccccggg atgttacgtc ctgtagaaac
       61 cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa
      121 ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt
      181 gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt
      241 ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg
      301 tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca
      361 tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag
      421 tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat
      481 ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta
      541 tgccggaatc catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat
      601 caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt
      661 ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg
      721 acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg
      781 ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct
      841 tcgcgtcggc atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc
      901 gttctacttt actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga
      961 taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg
     1021 tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt
     1081 ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc
     1141 gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc
     1201 gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat
     1261 gtggagtatt gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact
     1321 ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt
     1381 ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta
     1441 ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga
     1501 acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga
     1561 tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc
     1621 atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt
     1681 atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa
     1741 agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg
     1801 gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgag ctctagagca
     1861 tgccgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt ctccataata
     1921 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcacgtgtt
     1981 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     2041 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcacctaaag tccctataga
     2101 tctttgtcgt gaatataaac cagacatgag acgactaaac ctggagccca gacggaattc
     2161 ctaggtaaga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     2221 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc
     2281 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     2341 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     2401 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata
     2461 atctgcaccg gatctaccat ggggattgaa caagatggat tgcacgcagg ttctccggcc
     2521 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2581 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2641 tccggtgccc tgaatgaact ccaggacgag gcagcgcggc tatcgtggct ggccacgacg
     2701 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2761 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2821 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2881 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2941 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3001 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg
     3061 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3121 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3181 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3241 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggatcga
     3301 tcctctagct agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc
     3361 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     3421 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     3481 ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt
     3541 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     3601 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     3661 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     3721 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     3781 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     3841 gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     3901 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     3961 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     4021 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     4081 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     4141 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     4201 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     4261 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     4321 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     4381 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     4441 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     4501 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     4561 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     4621 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     4681 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     4741 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     4801 ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg
     4861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5041 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5701 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     5761 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     5821 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5881 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5941 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     6001 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     6061 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     6121 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     6181 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     6241 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     6301 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     6361 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     6421 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     6481 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     6541 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     6601 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     6661 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     6721 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     6781 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     6841 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     6901 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     6961 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     7021 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     7081 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     7141 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     7201 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     7261 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     7321 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     7381 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     7441 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     7501 gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
     7561 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
     7621 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
     7681 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
     7741 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
     7801 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
     7861 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
     7921 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
     7981 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
     8041 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
     8101 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
     8161 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
     8221 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
     8281 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
     8341 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
     8401 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
     8461 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
     8521 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
     8581 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
     8641 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
     8701 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
     8761 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
     8821 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
     8881 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
     8941 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
     9001 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
     9061 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
     9121 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
     9181 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
     9241 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
     9301 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
     9361 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
     9421 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
     9481 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
     9541 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
     9601 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
     9661 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
     9721 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
     9781 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct
     9841 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
     9901 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
     9961 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10021 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10081 cacgacgttg taaaacgacg gccagtgcca
//
