LOCUS       40924_8646        8006 bp DNA     linear   UNA 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    complement(1..56)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        180..363
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             381..1169
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1229..1403
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(1481..1505)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1930..2721
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2811..3399
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3585..3725)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4069..4263)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4332..5396)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(5833..6459)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(7759..7783)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     7986..8002
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcctag
       61 gtaagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
      121 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      181 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      241 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      301 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      361 gcaccggatc taccatgggg attgaacaag atggattgca cgcaggttct ccggccgctt
      421 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      481 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      541 gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      601 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      661 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      721 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      781 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      841 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      901 aggcgcgcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
      961 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     1021 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     1081 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     1141 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg gatcgatcct
     1201 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     1261 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     1321 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     1381 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     1441 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     1501 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     1561 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     1621 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     1681 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     1741 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     1801 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     1861 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     1921 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     1981 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     2041 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     2101 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     2161 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     2221 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     2281 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     2341 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     2401 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     2461 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     2521 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     2581 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     2641 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     2701 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
     2761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2941 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3601 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     3661 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     3721 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3781 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3841 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     3901 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     3961 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     4021 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     4081 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     4141 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     4201 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     4261 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     4321 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     4381 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     4441 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     4501 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     4561 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     4621 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     4681 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     4741 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     4801 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     4861 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     4921 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     4981 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     5041 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     5101 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     5161 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     5221 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     5281 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     5341 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca
     5401 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc
     5461 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     5521 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     5581 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     5641 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     5701 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     5761 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
     5821 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
     5881 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
     5941 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
     6001 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
     6061 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
     6121 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
     6181 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
     6241 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
     6301 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
     6361 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
     6421 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
     6481 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
     6541 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
     6601 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
     6661 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
     6721 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
     6781 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
     6841 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
     6901 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
     6961 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
     7021 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
     7081 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
     7141 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
     7201 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
     7261 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
     7321 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
     7381 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
     7441 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
     7501 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
     7561 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
     7621 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     7681 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccgt tattctaata
     7741 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
     7801 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag
     7861 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7921 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7981 acgttgtaaa acgacggcca gtgcca
//
