LOCUS       V008783                 6416 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008783
VERSION     V008783
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..1584
                     /gene="CAT"
                     /label="Catalase"
                     /note="Catalase from Homo sapiens. Accession#: P04040"
     polyA_signal    1773..1828
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2189..2205)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2213..2229)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2237..2267)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2281..2302)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2360..2556
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2562..2696
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2934..3522)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3696..4553)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4554..4658)
                     /label="AmpR promoter"
     regulatory      4686..5066
                     /label="hCMV enhancer"
                     /note="hCMV enhancer"
                     /regulatory_class="promoter"
     enhancer        4689..5068
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5070..5347
                     /label="chicken beta-actin promoter"
     intron          5348..6365
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcatggcgg acagccggga cccagccagc gaccagatga agcagtggaa ggagcagcgg
       61 gcccctcaga aacccgatgt cctgaccacc ggaggcggga acccaatagg agataaactt
      121 aatatcatga ctgcggggcc ccgagggccc ctcctcgttc aagatgtggt tttcaccgac
      181 gagatggcac actttgacag agagcggatt cctgagagag tggtacatgc aaagggagca
      241 ggtgcttttg gatactttga ggtcacccac gatattacca gatactccaa ggcaaaggtg
      301 tttgagcata ttgggaagag gactcctatt gccgtccgat tctccacagt cgctggagag
      361 tcaggctcag ctgacacagt tcgggaccct cgtgggtttg cagtgaaatt ttacacagaa
      421 gatggtaact gggatctcgt tggaaataac acccccattt tcttcatcag ggatcccata
      481 ttgtttccat cttttatcca cagccaaaag agaaatcctc agacacatct gaaggatccg
      541 gacatggtct gggacttctg gagcctacgt cctgagtctc tgcatcaggt ttctttcttg
      601 ttcagtgatc gggggattcc agatggacat cgccacatga atggatatgg atcacatact
      661 ttcaagctgg ttaatgcaaa tggggaggca gtttattgca aattccatta taagactgac
      721 cagggcatca aaaacctttc tgttgaagat gcggcgagac tttcccagga agatcctgac
      781 tatggcatcc gggatctttt taacgccatt gccacaggaa agtacccctc ctggactttt
      841 tacatccagg tcatgacatt taatcaggca gaaacttttc catttaatcc attcgatctc
      901 accaaggttt ggcctcacaa ggactaccct ctcatcccag ttggtaaact ggtcttaaac
      961 cggaatccag ttaattactt tgctgaggtt gaacagatag ccttcgaccc aagcaacatg
     1021 ccacctggca ttgaggccag tcctgacaaa atgcttcagg gccgcctttt tgcctatcct
     1081 gacactcacc gccatcgcct gggacccaat tatcttcata tacctgtgaa ctgtccctac
     1141 cgtgctcgag tggccaacta ccaacgtgac ggcccgatgt gcatgcagga caatcagggt
     1201 ggtgctccaa attactaccc caacagcttt ggtgctccgg aacaacagcc ttctgccttg
     1261 gagcacagca tccaatattc tggagaagtg cggagattca acactgccaa tgatgataac
     1321 gttactcagg tgcgggcatt ctatgtgaac gtgctgaatg aggaacagag gaaacgtctg
     1381 tgtgagaaca ttgccggcca cctgaaggat gcacaaattt tcatccagaa gaaagcggtc
     1441 aagaacttca ctgaggtcca ccctgactac gggagccaca tccaggctct tctggacaag
     1501 tacaatgctg agaagcctaa gaatgcgatt cacacctttg tgcagtccgg atctcacttg
     1561 gcggcaaggg agaaggcaaa tctgtgaggc cggggccctg cacctgtgca tgaagctnnn
     1621 aagctaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     1681 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     1741 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     1801 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     1861 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     1921 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     1981 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     2041 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     2101 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     2161 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2221 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta
     2281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2341 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2401 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2461 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     2521 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     2581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     2701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2821 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga
     2881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3361 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3421 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4141 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4321 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4501 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4561 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4681 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     4741 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     4801 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4861 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4921 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4981 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5041 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     5101 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     5161 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga
     5221 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     5281 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     5341 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg
     5401 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     5461 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     5521 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     5581 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     5641 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     5701 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     5761 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     5821 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     5881 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     5941 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga
     6001 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     6061 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     6121 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     6181 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     6241 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     6301 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     6361 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa
//
