LOCUS       40924_8591        5620 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGSpPHGPx, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5620)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5620)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..595
                     /label=pPHGPx
                     /note="pPHGPx"
     misc_feature    596..814
                     /label=3' UTR of pPHGPx
                     /note="3' UTR of pPHGPx"
     polyA_signal    977..1032
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1393..1409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1417..1433)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1441..1471)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1485..1506)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1564..1760
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1766..1900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2138..2726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2900..3757)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3758..3862)
                     /label=AmpR promoter
     enhancer        3893..4272
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3893..4270
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4274..4551
                     /label=chicken beta-actin promoter
     intron          4552..5569
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gcgggccccc cctcgagaac atatgtgcgc gtcccgcgac gactggcgat gtgctcgctc
      121 catgcacgaa ttctcagcca aggacatcga cgggcacatg gtgaacctgg acaagtaccg
      181 gggctacgtg tgcatcgtca ccaatgtggc ctctcaatga ggcaagacgg aggtaaacta
      241 cactcagctg gtcgacctgc acgcccgata tgctgagtgt ggtttacgga ttctggcctt
      301 cccttgcaac cagtttggga ggcaggagcc agggagtgat gctgagatca aagaatttgc
      361 tgctggctac aacgtcaaat ttgatatgtt cagcaagatc tgtgtgaatg gggacgatgc
      421 ccaccctctg tggaagtgga tgaaagtcca gcccaagggg aggggcatgc tgggaaatgc
      481 tatcaaatgg aactttacca agttcctcat tgataagaac ggctgtgtgg tgaagcggta
      541 cggtcccatg gaagagcccc aggtcataga gaaggacctg ccgtgctacc tctagctcaa
      601 caagtgtgtg ctgagcctgc tgcctgtgcc ctcagagcct tccacctggc acccatgaca
      661 gtctgcctaa aaaccagccc tggtggggca gactcgagaa cctggcgtgc aaccccgcca
      721 gaggaaggtc ccttggccca gcttgcagct cgcaccccac cccaggctgc cttgtgggaa
      781 taaaccatac aaattggcta aaaaaaaaaa aaaaacccga attcctgcag cccgggggat
      841 ccactagtaa cggccgccag tgtgctggaa ttctgcagat ccaatgccct ggctcacaaa
      901 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
      961 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1021 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1081 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1141 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1201 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1261 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1321 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     1381 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1441 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     1501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     1561 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1741 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     1801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1861 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     1921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2041 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     3901 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3961 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4021 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4081 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4141 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4201 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4261 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4321 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4381 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     4441 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     4501 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     4561 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     4621 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     4681 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     4741 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     4801 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     4861 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     4921 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     4981 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5041 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5101 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5161 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5221 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5281 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     5341 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     5401 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     5461 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     5521 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     5581 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//
