LOCUS       V008785                 5815 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008785
VERSION     V008785
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5815)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..21
                     /label="5UTR"
                     /note="5UTR"
     CDS             25..921
                     /gene="P35"
                     /label="Early 35 kDa protein"
                     /note="Early 35 kDa protein from Autographa californica
                     nuclear polyhedrosis virus. Accession#: P08160"
     misc_feature    925..1023
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    1172..1227
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1588..1604)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1612..1628)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1636..1666)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1680..1701)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1759..1955
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1961..2095
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2333..2921)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3095..3952)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3953..4057)
                     /label="AmpR promoter"
     enhancer        4088..4467
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4088..4465
                     /label="hCMV"
                     /note="hCMV"
     promoter        4469..4746
                     /label="chicken beta-actin promoter"
     intron          4747..5764
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcgatcttt accatagcaa aatgatgtgt gtaatttttc cggtagaaat cgacgtgtcc
       61 cagacgatta ttcgagattg tcaggtggac aaacaaacca gagagttggt gtacattaac
      121 aagattatga acacgcaatt gacaaaaccc gttctcatga tgtttaacat ttcgggtcct
      181 atacgaagcg ttacgcgcaa gaacaacaat ttgcgcgaca gaataaaatc aaaagtcgat
      241 gaacaatttg atcaactaga acgcgattac agcgatcaaa tggatggatt ccacgatagc
      301 atcaagtatt ttaaagatga acactattcg gtaagttgcc aaaatggcag cgtgttgaaa
      361 agcaagtttg ctaaaatttt aaagagtcat gattataccg ataaaaagtc tattgaagct
      421 tacgagaaat actgtttgcc caaattggtc gacgaacgca acgactacta cgtggcggta
      481 tgcgtgttga agccgggatt tgagaacggc agcaaccaag tgctatcttt cgagtacaac
      541 ccgattggta acaaagttat tgtgccgttt gctcacgaaa ttaacgacac gggactttac
      601 gagtacgacg tcgtagctta cgtggacagt gtgcagtttg atggcgaaca atttgaagag
      661 tttgtgcaga gtttaatatt gccgtcgtcg ttcaaaaatt cggaaaaggt tttatattac
      721 aacgaagcgt cgaaaaacaa aagcatgatc tacaaggctt tagagtttac tacagaatcg
      781 agctggggca aatccgaaaa gtataattgg aaaatttttt gtaacggttt tatttatgat
      841 aaaaaatcaa aagtgttgta tgttaaattg cacaatgtaa ctagtgcact caacaaaaat
      901 gtaatattaa acacaattaa ataaatgtta aaatttattg cctaatatta ttttgtcatt
      961 gcttgtcatt tattaatttg gatgatgtca tttgttttta aaattgaact ggctttacga
     1021 gtagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     1081 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     1141 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     1201 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     1261 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     1321 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     1381 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     1441 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     1501 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     1561 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa
     1681 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1741 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     2101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2221 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag
     2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4081 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     4501 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     4561 gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag
     4621 gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga
     4681 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     4741 cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc
     4801 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     4861 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     4921 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     4981 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     5041 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     5101 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     5161 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     5221 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     5281 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     5341 ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag
     5401 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     5461 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     5521 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     5581 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     5641 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     5701 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     5761 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
//
