LOCUS       V008787                 5633 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008787
VERSION     V008787
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5633)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..882
                     /gene="Il1b"
                     /label="Interleukin-1 beta"
                     /note="Interleukin-1 beta from Mus musculus. Accession#:
                     P10749"
     polyA_signal    990..1045
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1406..1422)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1430..1446)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1454..1484)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1498..1519)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1577..1773
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1779..1913
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2151..2739)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2913..3770)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3771..3875)
                     /label="AmpR promoter"
     enhancer        3906..4285
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3906..4283
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4287..4564
                     /label="chicken beta-actin promoter"
     intron          4565..5582
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gatcagcgct ccaccatggc aactgttcct gaactcaact gtgaaatgcc accttttgac
      121 agtgatgaga atgacctgtt ctttgaagtt gacggacccc aaaagatgaa gggctgcttc
      181 caaacctttg acctgggctg tcctgatgag agcatccagc ttcaaatctc gcagcagcac
      241 atcaacaaga gcttcaggca ggcagtatca ctcattgtgg ctgtggagaa gctgtggcag
      301 ctacctgtgt ctttcccgtg gaccttccag gatgaggaca tgagcacctt cttttccttc
      361 atctttgaag aagagcccat cctctgtgac tcatgggatg atgatgataa cctgctggtg
      421 tgtgacgttc ccattagaca actgcactac aggctccgag atgaacaact aaaaagcctc
      481 gtgctgtcgg acccatatga gctgaaagct ctccacctca atggacagaa tatcaaccaa
      541 caagtgatat tctccatgag ctttgtacaa ggagaaccaa gcaacgacaa aatacctgtg
      601 gccttgggcc tcaaaggaaa gaatctatac ctgtcctgtg taatgaaaga cggcacaccc
      661 accctgcagc tggagagtgt ggatcccaag caatacccaa agaagaagat ggaaaaacgg
      721 tttgtcttca acaagataga agtcaagagc aaagtggagt ttgagtctgc agagttcccc
      781 aactggtaca tcagcacctc acaagcagag cacaagcctg tcttcctggg aaacaacagt
      841 ggtcaggaca taattgactt caccatggaa tctgtgtctt cctaagcgct gatccaatgc
      901 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
      961 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     1021 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     1081 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     1141 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     1201 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     1261 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     1321 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     1381 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
     1501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1561 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     1621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     1681 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     1741 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     1801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     1921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2041 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
     2101 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2161 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2221 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2281 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2341 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2401 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2461 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2521 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2581 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2641 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2701 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2761 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2821 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2881 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2941 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3001 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3061 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3121 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3181 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3241 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3301 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3361 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3421 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3481 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3541 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3601 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3661 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3721 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3841 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3901 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     3961 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4021 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4081 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4141 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4201 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4261 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4321 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     4381 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     4441 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     4501 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     4561 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     4621 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     4681 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     4741 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     4801 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     4861 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     4921 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     4981 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5041 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5101 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5161 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     5221 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     5281 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     5341 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     5401 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     5461 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     5521 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     5581 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
//
