LOCUS       V008788                 7937 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008788
VERSION     V008788
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             94..3135
                     /gene="PARP1"
                     /label="Poly [ADP-ribose] polymerase 1"
                     /note="Poly [ADP-ribose] polymerase 1 from Homo sapiens.
                     Accession#: P09874"
     misc_feature    3139..3181
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    3294..3349
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3710..3726)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3734..3750)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3758..3788)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3802..3823)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3881..4077
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4083..4217
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4455..5043)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5217..6074)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6075..6179)
                     /label="AmpR promoter"
     enhancer        6210..6589
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6210..6587
                     /label="hCMV"
                     /note="hCMV"
     promoter        6591..6868
                     /label="chicken beta-actin promoter"
     intron          6869..7886
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gggggatcct ctagagtcga cctgcagggg aggatggcgg agtcttcgga taagctctat
      121 cgagtcgagt acgccaagag cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc
      181 aaggactcgc tccggatggc catcatggtg cagtcgccca tgtttgatgg aaaagtccca
      241 cactggtacc acttctcctg cttctggaag gtgggccact ccatccggca ccctgacgtt
      301 gaggtggatg ggttctctga gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg
      361 gaagctggag gagtgacagg caaaggccag gatggaattg gtagcaaggc agagaagact
      421 ctgggtgact ttgcagcaga gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg
      481 gagaagatag aaaagggcca ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca
      541 cagctaggca tgattgaccg ctggtaccat ccaggctgct ttgtcaagaa cagggaggag
      601 ctgggtttcc ggcccgagta cagtgcgagt cagctcaagg gcttcagcct ccttgctaca
      661 gaggataaag aagccctgaa gaagcagctc ccaggagtca agagtgaagg aaagagaaaa
      721 ggcgatgagg tggatggagt ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac
      781 aaggatagta agcttgaaaa agccctaaag gctcagaacg acctgatctg gaacatcaag
      841 gacgagctaa agaaagtgtg ttcaactaat gacctgaagg agctactcat cttcaacaag
      901 cagcaagtgc cttctgggga gtcggcgatc ttggaccgag tagctgatgg catggtgttc
      961 ggtgccctcc ttccctgcga ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat
     1021 tactgcactg gggacgtcac tgcctggacc aagtgtatgg tcaagacaca gacacccaac
     1081 cggaaggagt gggtaacccc aaaggaattc cgagaaatct cttacctcaa gaaattgaag
     1141 gttaaaaagc aggaccgtat attcccccca gaaaccagcg cctccgtggc ggccacgcct
     1201 ccgccctcca cagcctcggc tcctgctgct gtgaactcct ctgcttcagc agataagcca
     1261 ttatccaaca tgaagatcct gactctcggg aagctgtccc ggaacaagga tgaagtgaag
     1321 gccatgattg agaaactcgg ggggaagttg acggggacgg ccaacaaggc ttccctgtgc
     1381 atcagcacca aaaaggaggt ggaaaagatg aataagaaga tggaggaagt aaaggaagcc
     1441 aacatccgag ttgtgtctga ggacttcctc caggacgtct ccgcctccac caagagcctt
     1501 caggagttgt tcttagcgca catcttgtcc ccttgggggg cagaggtgaa ggcagagcct
     1561 gttgaagttg tggccccaag agggaagtca ggggctgcgc tctccaaaaa aagcaagggc
     1621 caggtcaagg aggaaggtat caacaaatct gaaaagagaa tgaaattaac tcttaaagga
     1681 ggagcagctg tggatcctga ttctggactg gaacactctg cgcatgtcct ggagaaaggt
     1741 gggaaggtct tcagtgccac ccttggcctg gtggacatcg ttaaaggaac caactcctac
     1801 tacaagctgc agcttctgga ggacgacaag gaaaacaggt attggatatt caggtcctgg
     1861 ggccgtgtgg gtacggtgat cggtagcaac aaactggaac agatgccgtc caaggaggat
     1921 gccattgagc acttcatgaa attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa
     1981 aatttcacga agtatcccaa aaagttctac cccctggaga ttgactatgg ccaggatgaa
     2041 gaggcagtga agaagctgac agtaaatcct ggcaccaagt ccaagctccc caagccagtt
     2101 caggacctca tcaagatgat ctttgatgtg gaaagtatga agaaagccat ggtggagtat
     2161 gagatcgacc ttcagaagat gcccttgggg aagctgagca aaaggcagat ccaggccgca
     2221 tactccatcc tcagtgaggt ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc
     2281 ctggatctct caaatcgctt ttacaccctg atcccccacg actttgggat gaagaagcct
     2341 ccgctcctga acaatgcaga cagtgtgcag gccaaggtgg aaatgcttga caacctgctg
     2401 gacatcgagg tggcctacag tctgctcagg ggagggtctg atgatagcag caaggatccc
     2461 atcgatgtca actatgagaa gctcaaaact gacattaagg tggttgacag agattctgaa
     2521 gaagccgaga tcatcaggaa gtatgttaag aacactcatg caaccacaca caatgcgtat
     2581 gacttggaag tcatcgatat ctttaagata gagcgtgaag gcgaatgcca gcgttacaag
     2641 ccctttaagc agcttcataa ccgaagattg ctgtggcacg ggtccaggac caccaacttt
     2701 gctgggatcc tgtcccaggg tcttcggata gccccgcctg aagcgcccgt gacaggctac
     2761 atgtttggta aagggatcta tttcgctgac atggtctcca agagtgccaa ctactgccat
     2821 acgtctcagg gagacccaat aggcttaatc ctgttgggag aagttgccct tggaaacatg
     2881 tatgaactga agcacgcttc acatatcagc aagttaccca agggcaagca cagtgtcaaa
     2941 ggtttgggca aaactacccc tgatccttca gctaacatta gtctggatgg tgtagacgtt
     3001 cctcttggga ccgggatttc atctggtgtg aatgacacct ctctactata taacgagtac
     3061 attgtctatg atattgctca ggtaaatctg aagtatctgc tgaaactgaa attcaatttt
     3121 aagacctccc tgtggtaatt gggagaggta gccgagtcac acccggtggc tctggtatga
     3181 attcgagctc ggtaccccca atgccctggc tcacaaatac cactgagatc tttttccctc
     3241 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     3301 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3361 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     3421 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     3481 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     3541 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     3601 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     3661 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3781 agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3841 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     3901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4081 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4201 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat
     4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6181 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta
     6241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     6601 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     6661 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc
     6721 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc
     6781 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     6841 ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc
     6901 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     6961 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     7021 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     7081 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     7141 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     7201 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     7261 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     7321 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     7381 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     7441 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     7501 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     7561 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     7621 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     7681 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     7741 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     7801 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     7861 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     7921 atcattttgg caaagaa
//
