LOCUS       V008789                 6345 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008789
VERSION     V008789
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6345)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6345)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..39
                     /label="5UTR"
                     /note="5UTR"
     CDS             40..705
                     /gene="SOD2"
                     /label="Superoxide dismutase [Mn], mitochondrial"
                     /note="Superoxide dismutase [Mn], mitochondrial from Homo
                     sapiens. Accession#: P04179"
     misc_feature    709..801
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    954..1009
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     polyA_signal    1359..1493
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1359..1433)
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      1359..1433
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     misc_feature    1489..2234
                     /label="PY"
                     /note="PY"
     rep_origin      2122..2234
                     /label="PY-O"
                     /note="PY-O"
     rep_origin      2336..2471
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    2491..2625
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3625..4482)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4483..4587)
                     /label="AmpR promoter"
     enhancer        4618..4997
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4618..4995
                     /label="hCMV"
                     /note="hCMV"
     promoter        4999..5276
                     /label="chicken beta-actin promoter"
     intron          5277..6294
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcggcggcg gcatcagcgg tagcaccagc actagcagca tgttgagccg ggcagtgtgc
       61 ggcaccagca ggcagctggc tccggctttg gggtatctgg gctccaggca gaagcacagc
      121 ctccccgacc tgccctacga ctacggcgcc ctggaacctc acatcaacgc gcagatcatg
      181 cagctgcacc acagcaagca ccacgcggcc tacgtgaaca acctgaacgt caccgaggag
      241 aagtaccagg aggcgttggc caagggagat gttacagccc agatagctct tcagcctgca
      301 ctgaagttca atggtggtgg tcatatcaat catagcattt tctggacaaa cctcagccct
      361 aacggtggtg gagaacccaa aggggagttg ctggaagcca tcaaacgtga ctttggttcc
      421 tttgacaagt ttaaggagaa gctgacggct gcatctgttg gtgtccaagg ctcaggttgg
      481 ggttggcttg gtttcaataa gcaacgggga cacttacaaa ttgctgcttg tccaaatcag
      541 gatccactgc aaggaacaac aggccttatt ccactgctgg ggattgatgt gtgggagcac
      601 gcttactacc ttcagtataa aaatgtcagg cctgattatc taaaagctat ttggaatgta
      661 atcaactggg agaatgtaac tgaaagatac atggcttgca aaaagtaaac cacgatcgtt
      721 atgctgatca taccctaatg atcccagcaa gataatgtcc tgtcttctaa gatgtgcatc
      781 aagcctggta catactgaaa cccccgaatt cactcctcag gtgcaggctg cctatcagaa
      841 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
      901 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
      961 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     1021 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     1081 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     1141 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     1201 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     1261 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     1321 tctcttatgg agatccctcg acctgcagcc caagcttgaa cttgtttatt gcagcttata
     1381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttatcactgc
     1441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcccgccat
     1501 ggtatcaacg ccatatttct atttacagta gggacctctt cgttgtgtag gtaccgctgt
     1561 attcctaggg aaatagtaga ggcaccttga actgtctgca tcagccatat agcccccgct
     1621 gttcgactta caaacacagg cacagtactg acaaacccat acacctcctc tgaaataccc
     1681 atagttgcta gggctgtctc cgaactcatt acaccctacc aagtgagagc tgtaatttcg
     1741 cgatcaaggg cagcgagggc ttctccagat aaaatagctt ctgccgagag tcccgtaagg
     1801 gtagacactt cagctaatcc ctcgatgagg tctactagaa tagtcagtgc ggctcccatt
     1861 ttgaaaattc acttacttga tcagcttcag aagatgggcg agggcctcca acacagtaat
     1921 tttcctcccg actcttaaaa tagaaaatgt caagtcagtt aaggaggaag tggactaact
     1981 gacgcagctg gccgtgcgac atcctctttt aattagttgc taggcaacgc cctccagagg
     2041 gcgtgtggtt ttgcaagagg aagcaaaagc ctctccaccc aggcctagaa tgtttccacc
     2101 caatcattac tatgacaaca gctgtttttt ttagtattaa gcagaggccg gggacccctg
     2161 ggcccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag aggcaacttg
     2221 tcaaaacagg actgcttcta tttctgtcac actgtctggc cctgtcacaa ggtccagcac
     2281 ctccataccc cctttaataa gcagtttggg aacgggtgcg ggtcttactc cgcccatccc
     2341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     2461 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     2521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2761 taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca
     2821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3601 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3661 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3721 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3781 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3841 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3901 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3961 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4021 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4081 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4141 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4201 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4261 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4321 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4381 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4441 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
     4621 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4681 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4741 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4801 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4861 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4921 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     4981 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     5041 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     5101 gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg
     5161 gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     5221 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     5281 tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
     5341 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
     5401 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
     5461 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
     5521 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
     5581 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     5641 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     5701 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     5761 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     5821 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     5881 cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc
     5941 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     6001 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     6061 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     6121 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     6181 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     6241 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     6301 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
//
