LOCUS       V008790                 5572 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008790
VERSION     V008790
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5572)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..752
                     /gene="BCL2"
                     /label="Apoptosis regulator Bcl-2"
                     /note="Apoptosis regulator Bcl-2 from Homo sapiens.
                     Accession#: P10415"
     polyA_signal    929..984
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1345..1361)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1369..1385)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1393..1423)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1437..1458)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1516..1712
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1718..1852
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2090..2678)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2852..3709)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3710..3814)
                     /label="AmpR promoter"
     enhancer        3845..4224
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3845..4222
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4226..4503
                     /label="chicken beta-actin promoter"
     intron          4504..5521
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcggcttgc ccccgttgct tttcctctgg gaaggatggc gcacgctggg agaacggggt
       61 acgataaccg ggagatagtg atgaagtaca tccattataa gctgtcgcag aggggctacg
      121 agtgggatgc gggagatgtg ggcgccgcgc ccccgggggc cgcccccgca ccgggcatct
      181 tctcctccca gcccgggcac acgccccatc cagccgcatc ccgcgacccg gtcgccagga
      241 cctcgccgct gcagaccccg gctgcccccg gcgccgccgc ggggcctgcg ctcagcccgg
      301 tgccacctgt ggtccacctg accctccgcc aggccggcga cgacttctcc cgccgctacc
      361 gccgcgactt cgccgagatg tccagccagc tgcacctgac gcccttcacc gcgcggggac
      421 gctttgccac ggtggtggag gagctcttca gggacggggt gaactggggg aggattgtgg
      481 ccttctttga gttcggtggg gtcatgtgtg tggagagcgt caaccgggag atgtcgcccc
      541 tggtggacaa catcgccctg tggatgactg agtacctgaa ccggcacctg cacacctgga
      601 tccaggataa cggaggctgg gatgcctttg tggaactgta cggccccagc atgcggcctc
      661 tgtttgattt ctcctggctg tctctgaaga ctctgctcag tttggccctg gtgggagctt
      721 gcatcaccct gggtgcctat ctgggccaca agtgaagtca acatgcctgc cccaaaagcc
      781 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
      841 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
      901 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
      961 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     1021 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     1081 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     1141 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     1201 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     1261 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     1321 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
     1441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1501 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     1561 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1621 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     1681 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     1741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     1861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1981 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
     2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3841 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3901 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3961 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4021 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4081 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4141 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4201 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     4261 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4321 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
     4381 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4441 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4501 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
     4561 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4621 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     4681 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     4741 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     4801 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     4861 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     4921 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     4981 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5041 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5101 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
     5161 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     5221 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     5281 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     5341 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     5401 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     5461 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     5521 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//
