LOCUS       40924_8531        5644 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGSbGPx, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5644)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    101..718
                     /label=bGPx
                     /note="bGPx"
     misc_feature    719..897
                     /label=3' UTR of bGPx
                     /note="3' UTR of bGPx"
     polyA_signal    1001..1056
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1417..1433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1441..1457)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1465..1495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1509..1530)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1588..1784
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1790..1924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2162..2750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2924..3781)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3782..3886)
                     /label=AmpR promoter
     enhancer        3917..4296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3917..4294
                     /label=hCMV
                     /note="hCMV"
     promoter        4298..4575
                     /label=chicken beta-actin promoter
     intron          4576..5593
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggatccnnng cgctctgctc tggattcgga aacggatacc atgtgcgccg ctcagcgctc
      121 ggcggccgcc ctggcggcgg cagccccgcg cacagtgtac gccttctccg cgcgccctct
      181 ggccggcggg gagcccttca acctgtcctc cctgcggggc aaggtgctgc tcattgagaa
      241 cgtagcatcg ctctgaggca caacggtgcg ggactacacc cagatgaatg acctgcagcg
      301 gcgccttgga ccccggggcc tggtcgtgct cggcttcccc tgcaaccagt ttgggcatca
      361 ggaaaacgcc aagaacgagg agatcctgaa ttgcctgaag tacgtccgac caggcggcgg
      421 gttcgagccc aactttatgc tcttcgaaaa gtgcgaggtg aatggcgaga aggcgcatcc
      481 gctcttcgcc ttccttcggg aggttctgcc cacgccaagt gacgacgcca ctgctctcat
      541 gaccgaccct aagttcatca cctggtcccc ggtgtgccgc aacgacgtct cctggaactt
      601 cgagaagttc ctggtgggcc cagacggtgt gcccgtgcgc aggtacagcc gccgctttct
      661 gaccatcgac atcgagcctg acattgaaac cctgctgtcc cagggggcct ctgcctaggg
      721 tgcccctccc acccttgctg cttggcggtc agcgctgctc tccagggatt ttgcccatga
      781 aggtgttccc tctaaaccta cgtggaggaa tgcctgatgt ccaggaaaat cccctgaggt
      841 gggcgctggt cctatccatc ccagcctccc ctttcccaga ccacagctcc tcatgacnng
      901 gatcccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
      961 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     1021 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     1081 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     1141 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     1201 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     1261 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     1321 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     1381 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1561 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     1621 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     1681 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     1741 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     1801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1921 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2041 tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga
     2101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3901 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3961 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4021 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4081 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4141 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4201 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4261 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     4321 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     4381 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg
     4441 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc
     4501 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     4561 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc
     4621 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     4681 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     4741 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     4801 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     4861 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     4921 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     4981 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     5041 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     5101 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     5161 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg
     5221 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     5281 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     5341 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     5401 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     5461 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5521 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     5581 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     5641 agaa
//
