LOCUS       V008786                 6430 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008786
VERSION     V008786
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6430)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6430)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..111
                     /codon_start=1
                     /note="unnamed protein product; MTS of hMnSOD"
                     /protein_id="SJL88166.1"
                     /translation="MLSRAVCGTSRQLAPVLGYLGSRQ"
     CDS             112..1644
                     /gene="CAT"
                     /label="Catalase"
                     /note="Catalase from Homo sapiens. Accession#: P04040"
     polyA_signal    1787..1842
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2203..2219)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2227..2243)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2251..2281)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2295..2316)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2374..2570
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2576..2710
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3710..4567)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4568..4672)
                     /label="AmpR promoter"
     enhancer        4703..5082
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4703..5080
                     /label="hCMV enhancer"
                     /note="hCMV enhancer"
                     /regulatory_class="promoter"
     promoter        5084..5361
                     /label="chicken beta-actin promoter"
     intron          5362..6379
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcggcggcg gcatcagcgg tagcaccagc actagcagca tgttgagccg ggcagtgtgc
       61 ggcaccagca ggcagctggc tccggttttg gggtatctgg gctccaggca gatggcggac
      121 agccgggacc cagccagcga ccagatgaag cagtggaagg agcagcgggc ccctcagaaa
      181 cccgatgtcc tgaccaccgg aggcgggaac ccaataggag ataaacttaa tatcatgact
      241 gcggggcccc gagggcccct cctcgttcaa gatgtggttt tcaccgacga gatggcacac
      301 tttgacagag agcggattcc tgagagagtg gtacatgcaa agggagcagg tgcttttgga
      361 tactttgagg tcacccacga tattaccaga tactccaagg caaaggtgtt tgagcatatt
      421 gggaagagga ctcctattgc cgtccgattc tccacagtcg ctggagagtc aggctcagct
      481 gacacagttc gggaccctcg tgggtttgca gtgaaatttt acacagaaga tggtaactgg
      541 gatctcgttg gaaataacac ccccattttc ttcatcaggg atcccatatt gtttccatct
      601 tttatccaca gccaaaagag aaatcctcag acacatctga aggatccgga catggtctgg
      661 gacttctgga gcctacgtcc tgagtctctg catcaggttt ctttcttgtt cagtgatcgg
      721 gggattccag atggacatcg ccacatgaat ggatatggat cacatacttt caagctggtt
      781 aatgcaaatg gggaggcagt ttattgcaaa ttccattata agactgacca gggcatcaaa
      841 aacctttctg ttgaagatgc ggcgagactt tcccaggaag atcctgacta tggcatccgg
      901 gatcttttta acgccattgc cacaggaaag tacccctcct ggacttttta catccaggtc
      961 atgacattta atcaggcaga aacttttcca tttaatccat tcgatctcac caaggtttgg
     1021 cctcacaagg actaccctct catcccagtt ggtaaactgg tcttaaaccg gaatccagtt
     1081 aattactttg ctgaggttga acagatagcc ttcgacccaa gcaacatgcc acctggcatt
     1141 gaggccagtc ctgacaaaat gcttcagggc cgcctttttg cctatcctga cactcaccgc
     1201 catcgcctgg gacccaatta tcttcatata cctgtgaact gtccctaccg tgctcgagtg
     1261 gccaactacc aacgtgacgg cccgatgtgc atgcaggaca atcagggtgg tgctccaaat
     1321 tactacccca acagctttgg tgctccggaa caacagcctt ctgccttgga gcacagcatc
     1381 caatattctg gagaagtgcg gagattcaac actgccaatg atgataacgt tactcaggtg
     1441 cgggcattct atgtgaacgt gctgaatgag gaacagagga aacgtctgtg tgagaacatt
     1501 gccggccacc tgaaggatgc acaaattttc atccagaaga aagcggtcaa gaacttcact
     1561 gaggtccacc ctgactacgg gagccacatc caggctcttc tggacaagta caatgctgag
     1621 aagcctaaga atgcgattca cacctgacct caggtgcagg ctgcctatca gaaggtggtg
     1681 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     1741 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     1801 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     1861 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     1921 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     1981 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     2041 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     2101 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     2161 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     2221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2281 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2341 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     2401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     2581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2701 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2761 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag
     2881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3361 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4681 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt
     4741 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4801 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4861 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4921 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     4981 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5041 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     5101 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     5161 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg
     5221 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag
     5281 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     5341 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc
     5401 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     5461 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     5521 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     5581 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     5641 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     5701 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     5761 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     5821 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     5881 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     5941 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg
     6001 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     6061 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     6121 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     6181 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     6241 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     6301 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     6361 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     6421 tggcaaagaa
//
