LOCUS       40924_8541        5800 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGSE6L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5800)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5800)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..61
                     /label=rGlBf
                     /note="rGlBf"
     misc_feature    160..178
                     /label=5' UTR of mIghg2b
                     /note="5' UTR of mIghg2b"
     CDS             563..880
                     /codon_start=1
                     /label=mIg-kappa-CL
                     /note="Mouse immunoglobulin kappa light chain constant
                     region"
                     /translation="ADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGS
                     ERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN
                     EC"
     misc_feature    884..1052
                     /label=3' UTR of mIghg2b
                     /note="3' UTR of mIghg2b"
     polyA_signal    1157..1212
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1573..1589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1597..1613)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1621..1651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1665..1686)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1744..1940
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1946..2080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2318..2906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3080..3937)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3938..4042)
                     /label=AmpR promoter
     enhancer        4073..4452
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4073..4450
                     /label=hCMV
                     /note="hCMV"
     promoter        4454..4731
                     /label=chicken beta-actin promoter
     intron          4732..5749
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gctagagaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt
      121 gatacgaaac gaagcattgg aattccggat ccggccaagc ttgggatcac acacagacat
      181 gagtgtgccc actcaggtcc tggggttgct gctgctgtgg cttacagatg ccagatgtga
      241 catccagatg actcagtctc cagcctccct atccgtttct gtgggagaat ctgtcaccat
      301 cacatgtcga gcaagtgaga atatttacag taatttagca tggtatcagc agaaacaggg
      361 aaaatctcct cagctccttg tctatgttgc gacaaagtta gtagatggtg tgccatcaag
      421 gttcagtggc agtggatcag gcacacagta ttccctcaag atcaacagcc tgcagtctga
      481 agattttggg agttattact gtcaacattt ttgggacact ccattcacgt tcggctcggg
      541 gacaaagttg gaaatgaaac gggctgatgc tgcaccaact gtatccatct tcccaccatc
      601 cagtgagcag ttaacatctg gaggtgcctc agtcgtgtgc ttcttgaaca acttctaccc
      661 caaagacatc aatgtcaagt ggaagattga tgggagtgaa cgacaaaatg gcgtcctgaa
      721 cagttggact gatcaggaca gcaaagacag cacctacagc atgagcagca ccctcacgtt
      781 gaccaaggac gagtatgaac gacataacag ctatacctgt gaggccactc acaagacatc
      841 aacttcaccc attgtcaaga gcttcaacag gaatgagtgt tagagacaaa ggtcctgaga
      901 cgccaccacc agctccccag ctccatccta tcttcccttc taaggtcttg gaggcttccc
      961 cacaagcgac ctaccactgt tgcggtgctc caaacctcct ccccacctcc ttctcctcct
     1021 cctccctttc cttccccccc cccccccccg tcgactctag ccaatgccct ggctcacaaa
     1081 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     1141 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1201 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1261 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1321 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1381 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1441 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1501 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     1561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1621 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     1681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     1741 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1921 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     1981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2041 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2221 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4081 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4141 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4201 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4261 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4321 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4381 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4441 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4501 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4561 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     4621 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     4681 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     4741 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     4801 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     4861 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     4921 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     4981 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     5041 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     5101 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     5161 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5221 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5281 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5341 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5401 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5461 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     5521 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     5581 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     5641 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     5701 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     5761 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//
