LOCUS       40924_8526        4805 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    158..213
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(574..590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(598..614)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(622..652)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(666..687)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        745..941
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    947..1081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1319..1907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2081..2938)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2939..3043)
                     /label=AmpR promoter
     enhancer        3078..3457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3078..3455
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        3459..3736
                     /label=chicken beta-actin promoter
     intron          3737..4754
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
      121 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
      181 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
      241 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
      301 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
      361 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
      421 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
      481 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
      541 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt
      661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      721 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
      781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
      961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1081 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3061 tgccacctgg gtcgatcgac attgattatt gactagttat taatagtaat caattacggg
     3121 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3181 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3241 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3301 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3361 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3421 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     3481 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     3541 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
     3601 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
     3661 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     3721 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
     3781 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     3841 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     3901 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     3961 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     4021 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     4081 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     4141 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     4201 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     4261 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     4321 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
     4381 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4441 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     4501 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     4561 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     4621 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     4681 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     4741 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     4801 aagaa
//
