LOCUS       40924_8496        8321 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-prepro-TSE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8321)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8321)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             428..694
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     CDS             698..2629
                     /codon_start=1
                     /note="unnamed protein product; mature TS"
                     /protein_id="SJL88095.1"
                     /translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNVD
                     GVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVIVK
                     GNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVSLKK
                     FFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKTWKFG
                     KGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVWGPSPK
                     SDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDE
                     NSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDSHLSSICT
                     PADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAERVRNGLKFA
                     GVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLDSSGGKKLLG
                     LSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGELLEGSGQTVVP
                     DGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFLSQDLIGTEAHM
                     DSSSDTKEL"
     misc_feature    2630..2668
                     /label=E-tag
                     /note="E-tag"
     polyA_signal    3678..3733
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4094..4110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4118..4134)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4142..4172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4186..4207)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4265..4461
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    4467..4601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4839..5427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5601..6458)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6459..6563)
                     /label=AmpR promoter
     regulatory      6591..6971
                     /label=hCMV enhancer
                     /note="hCMV enhancer"
                     /regulatory_class="promoter"
     enhancer        6594..6973
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6975..7252
                     /label=chicken beta-actin promoter
     intron          7253..8270
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gtaatgctta gcgcagtctc tctatcgctt ctgaaccccg gtgcacctgt gccgaaacgc
      121 aaatggggaa acacccgctt tttggatgat tatgcattgt ctccacattg tatgcttcca
      181 agattctggt gggaatactg ctgatagcct aacgttcatg atcaaaattt aactgttcta
      241 acccctactt gacagcaata tataaacaga aggaagctgc cctgtcttaa accttttttt
      301 ttatcatcat tattagctta ctttcataat tgcgactggt tccaattgac aagcttttga
      361 ttttaacgac ttttaacgac aacttgagaa gatcaaaaaa caactaatta ttcgaaggat
      421 ccaaacgatg agatttcctt caatttttac tgcagtttta ttcgcagcat cctccgcatt
      481 agctgctcca gtcaacacta caacagaaga tgaaacggca caaattccgg ctgaagctgt
      541 catcggttac tcagatttag aaggggattt cgatgttgct gttttgccat tttccaacag
      601 cacaaataac gggttattgt ttataaatac tactattgcc agcattgctg ctaaagaaga
      661 aggggtatct ctcgagaaaa gagaggctga agcttacgtg ctggcacccg gatcgagccg
      721 agttgagctg tttaagcgta agaattcgac ggtgccgttt gaagacaagg ccggcaaagt
      781 caccgagcgg gttgtccact cgttccgcct ccccgccctt gttaatgtgg acggggtgat
      841 ggttgccatc gcggacgctc gctacgacac atccaatgac aactccctca ttgatacggt
      901 ggcgaagtac agcgtggacg atggggagac gtgggagacc caaattgcca tcaagaacag
      961 ccgtgtatcg tctgtttctc gtgtggtgga tcccaccgtg attgtgaagg gcaacaagct
     1021 ttacgtcctg gttggaagct actatagttc gagaagctac tggtcgtcgc atggtgatgc
     1081 gagagactgg gatattctgc ttgccgttgg tgaggtcacg aagtccattg cgggcggcaa
     1141 gataactgcg agtatcaaat gggggagccc cgtgtcactg aagaagtttt ttccggcaga
     1201 aatggaaggc atgcacacaa atcaatttct tggcggcgcg ggtgttgcca ttgtggcgtc
     1261 caacgggaat cttgtgtacc ctgtgcaggt tacgaacaaa aggaagcaag ttttctccaa
     1321 gatcttctac tcggaagatg atgggaagac gtggaagttt gggaagggta ggagcgattt
     1381 tggctgctct gaacctgtgg cccttgagtg ggaggggaag ctcatcataa acacccgagt
     1441 tgactggaaa cgccgtctgg tgtacgagtc cagtgacatg gggaatacgt gggtggaggc
     1501 tgtcggcacg ctctcgcgtg tgtggggccc ctcaccaaaa tcggaccatc ccggcagtca
     1561 gagcagcttc actgccgtga ccatcgaagg aatgcgtgtg atgctcttca cacacccgct
     1621 gaattttaag ggaaggtggc tgcgcgaccg actgaacctc tggctgacgg ataaccagcg
     1681 catttataac gttgggcaag tatccattgg tgatgaaaat tccgcctaca gctccgtcct
     1741 gtacaaggat gataagctgt actgtttgca tgagatcaac acggacgagg tgtacagcct
     1801 tgtttttgca cgcctggttg gcgagctacg gatcattaaa tcagtgctgc gatcctggaa
     1861 gaattgggac agccacctgt ccagcatttg cacccctgct gatccagccg cttcgtcgtc
     1921 agagagtggt tgtggtcccg ctgtcaccac ggttggtctt gttggctttt tgtccggcaa
     1981 tgcctcccaa aacgtatggg aggatgcgta ccgctgcgtc aacgcaagca cggcaaatgc
     2041 ggagagggtt cggaacggtt tgaagtttgc gggggttggc ggaggggcgc tttggccggt
     2101 gagccagcag gggcagaatc agcggtatcg ttttgcaaac cacgcgttca cgctggtggc
     2161 gtcggtgacg attcacgagg ctccgagggc cgcgagtcct ttgctgggtg cgagcctgga
     2221 ctcttctggt ggcaaaaaac tcctggggct ctcgtacgac gagaagcacc agtggcagcc
     2281 aatatacgga tcaacgccgg tgacgccgac gggatcgtgg gagacgggta agaggtacca
     2341 cgtggttctt acggtggcga ataaaattgg ctccgtgtac attgatggag aacttctgga
     2401 gggttcagga cagaccgttg tgccagacgg gaggacgcct gacatctccc acttctacgt
     2461 tggcgggtat ggaaggagtg atatgccaac cataagccac gtgacggtga ataatgttct
     2521 tctttacaac cgacagctga ataccgagga gatcaggacc ttgttcttga gccaggacct
     2581 gattggcacg gaagcacaca tggacagcag cagcgacacg aaggaattgg gtgcgccggt
     2641 gccgtatccg gatccgctgg aaccgcgtgc cgcatagact gttgaaagtt gtttagcaaa
     2701 acctcataca gaaaattcat ttactaacgt ctggaaagac gacaaaactt tagatcgtta
     2761 cgctaactat gagggctgtc tgtggaatgc tacaggcgtt gtggtttgta ctggtgacga
     2821 aactcagtgt tacggtacat gggttcctat tgggcttgct atccctgaaa atgagggtgg
     2881 tggctctgag ggtggcggtt ctgagggtgg cggttctgag ggtggcggta ctaaacctcc
     2941 tgagtacggt gatacaccta ttccgggcta tacttatatc aaccctctcg acggcactta
     3001 tccgcctggt actgagcaaa accccgctaa tcctaatcct tctcttgagg agtctcagcc
     3061 tcttaatact ttcatgtttc agaataatag gttccgaaat aggcagggtg cattaactgt
     3121 ttatacgggc actgttactc aaggcactga ccccgttaaa acttattacc agtacactcc
     3181 tgtatcatca aaagccatgt atgacgctta ctggaacggt aaattcagag actgcgcttt
     3241 ccattctggc tttaatgagg atccattcgt ttgtgaatat caaggccaat cgtctgacct
     3301 gcctcaacct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga
     3361 gggtggcggc tctgagggtg gcggttctga gggtggcggc tctgagggtg gcggttccgg
     3421 tggcggctcc ggttccggtg attttgatta tgaaaaaatg gcaaacgcta ataagggggc
     3481 tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc
     3541 tgtcgctact gattacggtg ctgctatcgg gccgcgaatt accaatgccc tggctcacaa
     3601 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     3661 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3721 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     3781 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     3841 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     3901 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     3961 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     4021 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     4081 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4141 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact
     4201 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     4261 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4441 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     4501 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4561 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     4621 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4681 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4741 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4801 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4861 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4921 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4981 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5041 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5101 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5161 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5221 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5281 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5341 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5401 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5461 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5521 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5581 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5641 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5701 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5761 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5821 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5881 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5941 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6001 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6061 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6121 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6181 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6241 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6301 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6361 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6421 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6481 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6541 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     6601 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6721 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6781 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6841 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6901 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6961 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     7021 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     7081 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     7141 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     7201 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
     7261 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
     7321 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     7381 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
     7441 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     7501 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
     7561 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
     7621 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     7681 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
     7741 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
     7801 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     7861 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
     7921 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     7981 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     8041 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     8101 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     8161 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     8221 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     8281 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//
