LOCUS       V008804                 6245 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008804
VERSION     V008804
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..832
                     /gene="IL1B"
                     /label="Interleukin-1 beta"
                     /note="Interleukin-1 beta from Homo sapiens. Accession#:
                     P01584"
     misc_feature    836..1436
                     /label="3' UTR of hIL1B"
                     /note="3' UTR of hIL1B"
     gap             1437..1453
                     /estimated_length=17
     polyA_signal    1602..1657
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2018..2034)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2042..2058)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2066..2096)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2110..2131)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2189..2385
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2391..2525
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2763..3351)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3525..4382)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4383..4487)
                     /label="AmpR promoter"
     enhancer        4518..4897
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4518..4895
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4899..5176
                     /label="chicken beta-actin promoter"
     intron          5177..6194
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattccgaag cagccatggc agaagtacct gagctcgcca gtgaaatgat
       61 ggcttattac agtggcaatg aggatgactt gttctttgaa gctgatggcc ctaaacagat
      121 gaagtgctcc ttccaggacc tggacctctg ccctctggat ggcggcatcc agctacgaat
      181 ctccgaccac cactacagca agggcttcag gcaggccgcg tcagttgttg tggccatgga
      241 caagctgagg aagatgctgg ttccctgccc acagaccttc caggagaatg acctgagcac
      301 cttctttccc ttcatctttg aagaagaacc tatcttcttc gacacatggg ataacgaggc
      361 ttatgtgcac gatgcacctg tacgatcact gaactgcacg ctccgggact cacagcaaaa
      421 aagcttggtg atgtctggtc catatgaact gaaagctctc cacctccagg gacaggatat
      481 ggagcaacaa gtggtgttct ccatgtcctt tgtacaagga gaagaaagta atgacaaaat
      541 acctgtggcc ttgggcctca aggaaaagaa tctgtacctg tcctgcgtgt tgaaagatga
      601 taagcccact ctacagctgg agagtgtaga tcccaaaaat tacccaaaga agaagatgga
      661 aaagcgattt gtcttcaaca agatagaaat caataacaag ctggaatttg agtctgccca
      721 gttccccaac tggtacatca gcacctctca agcagaaaac atgcccgtct tcctgggagg
      781 gaccaaaggc ggccaggata taactgactt caccatgcaa tttgtgtctt cctaaagaga
      841 gctgtaccca gagagtcctg tgctgaatgt ggactcaatc cctagggctg gcagaaaggg
      901 aacagaaagg tttttgagta cggctatagc ctggactttc ctgttgtcta caccaatgcc
      961 caactgcctg ccttagggta gtgctaagag gatctcctgt ccatcagcca ggacagtcag
     1021 ctctctcctt tcagggccaa tccccagccc ttttgttgag ccaggcctct ctcacctctc
     1081 ctactcactt aaagcccgcc tgacagaaac cacggccaca tttggttcta agaaaccctc
     1141 tgtcattcgc tcccacattc tgatgagcaa ccgcttccct atttatttat ttatttgttt
     1201 gtttgtttta ttcattggtc taatttattc aaagggggca agaagtagca gtgtctgtaa
     1261 aagagcctag tttttaatag ctatggaatc aattcaattt ggactggtgt gctctcttta
     1321 aatcaagtcc tttaattaag actgaaaata tataagctca gattatttaa atgggaatat
     1381 ttataaatga gcaaatatca tactgttcaa tggttctgaa ataaacttct ctgaagnnnn
     1441 nnnnnnnnnn nnngaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     1501 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     1561 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     1621 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     1681 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     1741 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     1801 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     1861 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     1921 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     1981 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2161 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     2221 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2281 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2341 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     2401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2521 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag
     2701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4501 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     4561 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4621 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4681 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4741 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4861 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     4921 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     4981 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
     5041 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
     5101 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     5161 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
     5221 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     5281 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     5341 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     5401 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     5461 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     5521 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     5581 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     5641 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     5701 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     5761 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
     5821 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     5881 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     5941 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     6001 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     6061 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     6121 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     6181 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     6241 aagaa
//
