LOCUS       V008805                 5929 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008805
VERSION     V008805
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5929)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5929)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(62..72)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(76..984)
                     /gene="Casp7"
                     /label="Caspase-7"
                     /note="Caspase-7 from Mus musculus. Accession#: P97864"
     misc_feature    complement(985..1173)
                     /label="5UTR"
                     /note="5UTR"
     polyA_signal    1286..1341
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1702..1718)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1726..1742)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1750..1780)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1794..1815)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1873..2069
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2075..2209
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2447..3035)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3209..4066)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4067..4171)
                     /label="AmpR promoter"
     enhancer        4202..4581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4202..4579
                     /label="hCMV"
                     /note="hCMV"
     promoter        4583..4860
                     /label="chicken beta-actin promoter"
     intron          4861..5878
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gctgaagggt ggtcaacggc tgaagtacag ctctttggtg agcatggaca ccatacacgg
      121 gatctgcttc ttctcgttga agcgtggatc atcagactgg gactcgaagt gcctggccac
      181 cctgtcgttc accctggtca ggatctgcat gatctcgagg tccttgccat gctcattcag
      241 gatggagcag agggcctgca caaaccagga gcctttccct gggttcctcc atgagtaata
      301 acctggaacc gtggagtaag caaagaggaa gtcggcttcc accgggatct tgttgcgggg
      361 attagcgtca atgtcgttga tgggccccga gtcagcctgg attccatcat cgagctccgt
      421 ccctcggcat gcctgaatga agaagagttt gggtttctct aacagggttt tgcatcggtc
      481 tcccctaaaa tgagctgtca gatcctttat gggtgtcacg ccatctttcc cgtaaatcag
      541 gtcctcttcc ccgtggctca gcaggacgca ggcgaagcag gccgagttgc tgtggtcctc
      601 ctcagaggct tttctaagca gatcttgcat ctttgcacaa gagcagtcat tgtggacggt
      661 tacttcaaaa cccaggtttt ggaagcactt gaagagggcc cctgcatctt tgtccgtccc
      721 attccggacg tccatacctg tcgctttgtc gaagttcttg ttgtttatga tgatgcattt
      781 acccatcttc tggaaatcca tgcggtacag ataagtgggc actcggtccc ggccggtcct
      841 gacggggccc gcagaggcat ttctcttctt cttcaagaga atagaggaga tgatagagga
      901 gcggtctggc ttggcgtcaa ctccgtcttc gctggaagaa tccacctttt ccagctccgc
      961 agcacagtcc tgatcatcgg tcatcgttcc cacggcggcg gccaggagcc cacggcagcg
     1021 tccgtcctcc gcaagggtcg agggcagctt tgggaaaact gaaagcaaaa gtcccggagg
     1081 cggaggggga ggagcgggcg ggccggacgg ggctgtgcct gcagcccggg aaagctgggg
     1141 gcgagcggga acgcgcgcta cggagcccgc cgcctcgtgc cgaattcccc caatgccctg
     1201 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     1261 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     1321 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     1381 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     1441 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     1501 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     1561 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     1621 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     1681 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg
     1801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1861 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2041 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     2101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     2221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2341 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag
     2401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
     4201 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4261 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4321 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4381 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4441 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4501 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4561 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     4621 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     4681 atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg
     4741 ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     4801 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     4861 ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     4921 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     4981 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
     5041 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
     5101 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
     5161 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     5221 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     5281 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     5341 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     5401 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     5461 gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg
     5521 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     5581 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     5641 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     5701 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     5761 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     5821 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     5881 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//
