LOCUS       V008808                 6040 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008808
VERSION     V008808
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6040)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..35
                     /label="5UTR"
                     /note="5UTR"
     CDS             36..863
                     /gene="Casp6"
                     /label="Caspase-6"
                     /note="Caspase-6 from Mus musculus. Accession#: O08738"
     misc_feature    867..1240
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    1397..1452
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1813..1829)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1837..1853)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1861..1891)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1905..1926)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1984..2180
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2186..2320
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2558..3146)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3320..4177)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4178..4282)
                     /label="AmpR promoter"
     enhancer        4313..4692
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4313..4690
                     /label="hCMV"
                     /note="hCMV"
     promoter        4694..4971
                     /label="chicken beta-actin promoter"
     intron          4972..5989
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcggcacga gctcgcgggc aggtgaaagt aaaacatgac agaaaccgat ggcttctaca
       61 aaagtaggga agtgttcgat ccagccgagc agtacaagat ggaccacaag aggagaggag
      121 ttgccctcat cttcaatcac gagaggttct tttggcacct gaccctccca gagaggcggg
      181 gcaccaacgc agacagagac aacctgactc gcaggttttc agatctagga tttgaagtga
      241 aatgctttaa cgacctcaga gcagaagaac tcctgctcaa aattcacgag gtgtcgactt
      301 caagccacat agatgccgat tgcttcatct gtgtcttcct gagccatggg gaaggcaacc
      361 acgtttacgc atacgacgcc aaaattgaaa ttcagacgtt gactggcttg ttcaaaggag
      421 acaagtgtca gagcctggtt ggaaaaccca agatatttat catccaggcc tgtcggggta
      481 gccagcatga cgtacccgtg gttcccctgg acatggtgga tcaccagaca gacaagctgg
      541 acaacgtgac ccaggtggat gctgcgtccg tgtacacgct gccggcaggg gcagacttcc
      601 tcatgtgcta ctctgtcgca gaagggtatt actctcaccg agaaactgtg aatggctcct
      661 ggtacattca ggatttgtgt gagatgctgg cgaggtacgg cagttccctg gagttcacgg
      721 agctgctcac gctggtgaac agaaaggtct ctcagcgccg cgtggacttc tgcaaagacc
      781 cggatgcaat cggcaagaag caggtgccct gcttcgcctc aatgctgacc aaaaagctgc
      841 atttctgtcc caaacctagc aagtagggcc atctgtcttg ctacatatat ttcacacaca
      901 tttccctttt tatacaaagg ccagctggtg gaagactttc acccatcaat gtaaactgct
      961 tcaattttgt agatgccata gaaaagtaat gttttatatt tgttactttt caatcatgtt
     1021 ggacgtggtg gaagggctaa aaataatcct caggaaatta ctatgcatca atgtctacta
     1081 catttattac atttttagta aactgtaaga ttactctaaa taccaactac tttgcctccc
     1141 cttttgtgac actgtttata tgagtttact gtataatgtt tgtaaagact tttgaaactc
     1201 tagactgaga ttgttttcgc gtaataaaat gtctaagaac ctcgtgccga attcactcct
     1261 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     1321 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     1381 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1441 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1501 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1561 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1621 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1681 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1741 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     1801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1861 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     1981 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2161 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     2221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2281 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     2341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2461 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4741 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     4801 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     4861 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     4921 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     4981 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     5041 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     5101 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     5161 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     5221 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     5281 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     5341 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     5401 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5461 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5521 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5581 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5641 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5701 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     5761 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     5821 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     5881 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     5941 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     6001 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//
