LOCUS       V008811                 8408 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008811
VERSION     V008811
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8408)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8408)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(182..2200)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(2204..3559)
                     /gene="Casp2"
                     /label="Caspase-2"
                     /note="Caspase-2 from Mus musculus. Accession#: P29594"
     misc_feature    complement(3560..3644)
                     /label="5UTR"
                     /note="5UTR"
     polyA_signal    3765..3820
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4181..4197)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4205..4221)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4229..4259)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4273..4294)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4352..4548
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4554..4688
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4926..5514)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5688..6545)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6546..6650)
                     /label="AmpR promoter"
     enhancer        6681..7060
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6681..7058
                     /label="hCMV"
                     /note="hCMV"
     promoter        7062..7339
                     /label="chicken beta-actin promoter"
     intron          7340..8357
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gcgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
      121 atctattcga tgatgaagat accccaccaa acccaaaaaa agagatcgaa ttcggcacga
      181 gatcaacatt tatttggcac ctgatggcaa tacaaaatct tggcagtggg aagtgtgaag
      241 attctaccac ttatgcatcc tatactgacc caaaaggcag aatttggcag caagtcttca
      301 aaccacttgg ttcatacaac aaacattagt tgagcacctc aactaccata ggcctcaaat
      361 ttgctgggca ctatggagat gtgaaagaag ttgtctcaca cgtgatatgt agaaaatata
      421 aaaaaacaac aacaaaacag tggataaaaa taaaacacct ggaaaaaaaa acccatatac
      481 taattgaact gtacactata atagcaattt agaaactgca aaaggtaggc tctaactaag
      541 gttagctgct ggtctccttc ctttactcag gaaacattca tgaagaccta gtaagtgcca
      601 gttactggat atcagtaagt aagaagcaca gttcccactc tcttggagca ttcagcctca
      661 gagggaacaa gtgaaacaag caaaagtgcc attacagcct gcagtaagca caacagagac
      721 caacatgatg ccctcataag gaggcacagt agacagcatg ggcagcatgg tcaagaggcc
      781 tccgccagac tcaaaaatca gagactagcc aaaggagagt tcagaacacc tgcaagacgg
      841 agatgaggac ctaagagggg ctggagcata gtgagaaatc accatttaat gagatccaaa
      901 gggaaggtat gagccagatc tcgtaagctg ggtgtaagaa tttgcatttt agatgaagcg
      961 taacaggaag ctagtgttct aagcaagaga atgacatcac cagatatttt aaaaaccaaa
     1021 ctactctgtt acatggagaa taaattggaa gagaggaaaa aatggaaatg cagaaggttt
     1081 tcaagaataa gcaagtttca taagagcaga gacaagtgct tctaggttat atcacatatg
     1141 gtactatccc cactaaaaca cagagaaaca atgagaaggg agacgctgcc acagaaaacc
     1201 aaagaagcct cacgctgaag cagaaggcag aaagaataag caactacagt acagcgtaag
     1261 tggcaagaaa catcctacaa aggacggctt cacagacttg gcagaagcag cgaatggtga
     1321 aggtaaagag gggaaccagt acaaagcaag agagaccctg gaggccagcc gtcaagtaca
     1381 gtgtaagtgc atgcagaagc cacagtaagg aaggagagac atcctgatga cgggtgatag
     1441 tgtgagacac agcacactca gctgtcactg cattttcatg tagttatgcc cgataaggtt
     1501 cattcagtga cagttcaaac aaagcatgac cagggaaatt tctatgactt ccagtgtcat
     1561 ggcatacaga ccctaggctc tgttcaaagg ctgggtgcac tgaacacaaa ggaaccaatg
     1621 gaaaggctga gggactgtcc ttcacctagg gcctgaactt aaaggtatgc atagaaactt
     1681 cgcaagtagt aggatctcat ctgtcccttc atgacagatg aaaacaccta cattaaattg
     1741 ggacatcttc caaatctcca ctgtgggaaa aatgaggttg tagatatgga aaaaaaagga
     1801 gatgatctct gagactcctt aactgattta ggaaaaaaaa aaaaagctat gatccaagta
     1861 ctcctaagat gccttgggaa caaaaatatc acaaacattg agaaatccaa atgccctctt
     1921 cccacaataa tactgtgttc acaatgtcat tttctaggct gtagctaaaa tgcttctggt
     1981 aacacatcca ttcagagctg gctctatagg tagagaacac gacaccggag gagccatcct
     2041 tatgtttcat acccaaagac agagatggct gacaggaaac aagcctggga gaatctcata
     2101 tccctgagag ggtagacaga acaaaaacca ttctgaagag aagtctccat tgtgccccca
     2161 gtggtccagt ggcctccaac agcaggaata gcaggcggca tcacgtgggt gggtagcctg
     2221 ggaacaggta gagttgctgg cacagagtac tacagtactc agacatctcc ttgcatcggt
     2281 ggaattctgt gccaggggca tagccttcac gctccttgat aagggcattc accttaacaa
     2341 gcatgtcggc cacgtgcatg tcacaagctc tttcagagaa cacctgagtg agggcctcaa
     2401 tgtaccagga accccgtttg gtgttccgca tggcagcatt acctttaagg caagcatagc
     2461 cacatatcat gtctgagcga gtaggcagtc tcatcttcat caactcctct ttgccagcat
     2521 cactctcctc acatccaggg gattgtgtgt ggttctttcc atcttgctgg tcgacacctc
     2581 tatctgtctc atctccacga catgcttgga tgaagaacat ttttggcttg ttctgtagac
     2641 ttggacagtt agcattgtca aaaagtcgaa aaacctcttg gagctgaagc agtttgccat
     2701 ctacaccata gatgccacct tccacaccat gtgagaggag tgccactacg caggagtctg
     2761 tgacccggtg tgcaggtaac tgtgcaaaat tctgaagttt ctcttgcatt tcctgtgcgg
     2821 tctggtcatg tagcacatgg acattgtagc ccaaaagctt gaagagggtg actagagtag
     2881 tgtggtccac atcccctcca gagcggaatt ccaggtcttt ctctccagtg aagtgcacat
     2941 tgctcagcac cagtgccaag ccacggggct gagattgcaa cctataggcc agctggtagt
     3001 gtgcctggta aaactcagga gtgcatggct tcaccagaag acagggagga ccatcaccat
     3061 tatctaagga gtgttccgta gcatctgtgg ataggcggag ctgcttgtga ggagggcagg
     3121 actcacacac cgagaaaggg agacttgtgt catagtcaca gctcaacggt gggagtacgt
     3181 gctgaatatc tgagagggtt gtgagcagta agtcttccaa gtgaccctgc ctggtctccc
     3241 gcagggcttc acagaaggca tcaaaagcct ggggtcctct ctttggcagc aggttgagga
     3301 gttccacatt ctggctgaaa ctgccccctt tggcctggat gagctccctc atttccaaag
     3361 tgataatgtc cttctctagg aggtgttcta acagctcgct cagcagcagc tgcttggcca
     3421 gcaccactcg attctttttc agtgtttcct ggtggtcagg atgcattcca cacactgcca
     3481 aaatcctgct cctcctgtca gccgccatca gccccttcct gtgcagggag gactgcgacc
     3541 tcccgctcgg cgccgccatt tccagctttt cccgggctgc acacgcacca cccacaaaac
     3601 aaacaaacgc tgggccgcgc cagcccccgc atccctcccc cagccctcgt gccgaattcc
     3661 cggggatccc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     3721 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3781 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3841 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     3901 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     3961 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4021 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4081 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4141 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4261 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4321 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     4381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     4561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4681 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     4861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6661 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     6721 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     6781 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     6841 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     6901 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6961 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     7021 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     7081 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     7141 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     7201 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     7261 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     7321 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     7381 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     7441 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     7501 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     7561 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     7621 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     7681 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     7741 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     7801 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     7861 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     7921 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     7981 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     8041 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     8101 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     8161 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     8221 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     8281 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     8341 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     8401 gcaaagaa
//
