LOCUS       V008812                 8408 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008812
VERSION     V008812
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8408)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8408)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    88..170
                     /label="5' UTR of mCASP-2"
                     /note="5' UTR of mCASP-2"
     CDS             171..1526
                     /gene="Casp2"
                     /label="Caspase-2"
                     /note="Caspase-2 from Mus musculus. Accession#: P29594"
     misc_feature    1530..3548
                     /label="3' UTR of mCASP-2"
                     /note="3' UTR of mCASP-2"
     polyA_signal    3765..3820
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4181..4197)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4205..4221)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4229..4259)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4273..4294)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4352..4548
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4554..4688
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4926..5514)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5688..6545)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6546..6650)
                     /label="AmpR promoter"
     enhancer        6681..7060
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      6681..7058
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        7062..7339
                     /label="chicken beta-actin promoter"
     intron          7340..8357
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggatccccgg gaattcggca cgagggctgg gggagggatg cgggggctgg cgcggcccag
      121 cgtttgtttg ttttgtgggt ggtgcgtgtg cagcccggga aaagctggaa atggcggcgc
      181 cgagcgggag gtcgcagtcc tccctgcaca ggaaggggct gatggcggct gacaggagga
      241 gcaggatttt ggcagtgtgt ggaatgcatc ctgaccacca ggaaacactg aaaaagaatc
      301 gagtggtgct ggccaagcag ctgctgctga gcgagctgtt agaacacctc ctagagaagg
      361 acattatcac tttggaaatg agggagctca tccaggccaa agggggcagt ttcagccaga
      421 atgtggaact cctcaacctg ctgccaaaga gaggacccca ggcttttgat gccttctgtg
      481 aagccctgcg ggagaccagg cagggtcact tggaagactt actgctcaca accctctcag
      541 atattcagca cgtactccca ccgttgagct gtgactatga cacaagtctc cctttctcgg
      601 tgtgtgagtc ctgccctcct cacaagcagc tccgcctatc cacagatgct acggaacact
      661 ccttagataa tggtgatggt cctccctgtc ttctggtgaa gccatgcact cctgagtttt
      721 accaggcaca ctaccagctg gcctataggt tgcaatctca gccccgtggc ttggcactgg
      781 tgctgagcaa tgtgcacttc actggagaga aagacctgga attccgctct ggaggggatg
      841 tggaccacac tactctagtc accctcttca agcttttggg ctacaatgtc catgtgctac
      901 atgaccagac cgcacaggaa atgcaagaga aacttcagaa ttttgcacag ttacctgcac
      961 accgggtcac agactcctgc gtagtggcac tcctctcaca tggtgtggaa ggtggcatct
     1021 atggtgtaga tggcaaactg cttcagctcc aagaggtttt tcgacttttt gacaatgcta
     1081 actgtccaag tctacagaac aagccaaaaa tgttcttcat ccaagcatgt cgtggagatg
     1141 agacagatag aggtgtcgac cagcaagatg gaaagaacca cacacaatcc cctggatgtg
     1201 aggagagtga tgctggcaaa gaggagttga tgaagatgag actgcctact cgctcagaca
     1261 tgatatgtgg ctatgcttgc cttaaaggta atgctgccat gcggaacacc aaacggggtt
     1321 cctggtacat tgaggccctc actcaggtgt tctctgaaag agcttgtgac atgcacgtgg
     1381 ccgacatgct tgttaaggtg aatgccctta tcaaggagcg tgaaggctat gcccctggca
     1441 cagaattcca ccgatgcaag gagatgtctg agtactgtag tactctgtgc cagcaactct
     1501 acctgttccc aggctaccca cccacgtgat gccgcctgct attcctgctg ttggaggcca
     1561 ctggaccact gggggcacaa tggagacttc tcttcagaat ggtttttgtt ctgtctaccc
     1621 tctcagggat atgagattct cccaggcttg tttcctgtca gccatctctg tctttgggta
     1681 tgaaacataa ggatggctcc tccggtgtcg tgttctctac ctatagagcc agctctgaat
     1741 ggatgtgtta ccagaagcat tttagctaca gcctagaaaa tgacattgtg aacacagtat
     1801 tattgtggga agagggcatt tggatttctc aatgtttgtg atatttttgt tcccaaggca
     1861 tcttaggagt acttggatca tagctttttt tttttttcct aaatcagtta aggagtctca
     1921 gagatcatct cctttttttt ccatatctac aacctcattt ttcccacagt ggagatttgg
     1981 aagatgtccc aatttaatgt aggtgttttc atctgtcatg aagggacaga tgagatccta
     2041 ctacttgcga agtttctatg cataccttta agttcaggcc ctaggtgaag gacagtccct
     2101 cagcctttcc attggttcct ttgtgttcag tgcacccagc ctttgaacag agcctagggt
     2161 ctgtatgcca tgacactgga agtcatagaa atttccctgg tcatgctttg tttgaactgt
     2221 cactgaatga accttatcgg gcataactac atgaaaatgc agtgacagct gagtgtgctg
     2281 tgtctcacac tatcacccgt catcaggatg tctctccttc cttactgtgg cttctgcatg
     2341 cacttacact gtacttgacg gctggcctcc agggtctctc ttgctttgta ctggttcccc
     2401 tctttacctt caccattcgc tgcttctgcc aagtctgtga agccgtcctt tgtaggatgt
     2461 ttcttgccac ttacgctgta ctgtagttgc ttattctttc tgccttctgc ttcagcgtga
     2521 ggcttctttg gttttctgtg gcagcgtctc ccttctcatt gtttctctgt gttttagtgg
     2581 ggatagtacc atatgtgata taacctagaa gcacttgtct ctgctcttat gaaacttgct
     2641 tattcttgaa aaccttctgc atttccattt tttcctctct tccaatttat tctccatgta
     2701 acagagtagt ttggttttta aaatatctgg tgatgtcatt ctcttgctta gaacactagc
     2761 ttcctgttac gcttcatcta aaatgcaaat tcttacaccc agcttacgag atctggctca
     2821 taccttccct ttggatctca ttaaatggtg atttctcact atgctccagc ccctcttagg
     2881 tcctcatctc cgtcttgcag gtgttctgaa ctctcctttg gctagtctct gatttttgag
     2941 tctggcggag gcctcttgac catgctgccc atgctgtcta ctgtgcctcc ttatgagggc
     3001 atcatgttgg tctctgttgt gcttactgca ggctgtaatg gcacttttgc ttgtttcact
     3061 tgttccctct gaggctgaat gctccaagag agtgggaact gtgcttctta cttactgata
     3121 tccagtaact ggcacttact aggtcttcat gaatgtttcc tgagtaaagg aaggagacca
     3181 gcagctaacc ttagttagag cctacctttt gcagtttcta aattgctatt atagtgtaca
     3241 gttcaattag tatatgggtt tttttttcca ggtgttttat ttttatccac tgttttgttg
     3301 ttgttttttt atattttcta catatcacgt gtgagacaac ttctttcaca tctccatagt
     3361 gcccagcaaa tttgaggcct atggtagttg aggtgctcaa ctaatgtttg ttgtatgaac
     3421 caagtggttt gaagacttgc tgccaaattc tgccttttgg gtcagtatag gatgcataag
     3481 tggtagaatc ttcacacttc ccactgccaa gattttgtat tgccatcagg tgccaaataa
     3541 atgttgatct cgtgccgaat tcgatctctt tttttgggtt tggtggggta tcttcatcat
     3601 cgaatagata gttatataca tcatccattg tagtggtatt aaacatccct gtagtgattc
     3661 caaacgcgcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     3721 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3781 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3841 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     3901 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     3961 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4021 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4081 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4141 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4261 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4321 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     4381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     4561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4681 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     4861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6661 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     6721 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     6781 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     6841 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     6901 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6961 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     7021 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     7081 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     7141 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     7201 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     7261 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     7321 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     7381 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     7441 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     7501 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     7561 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     7621 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     7681 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     7741 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     7801 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     7861 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     7921 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     7981 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     8041 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     8101 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     8161 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     8221 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     8281 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     8341 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     8401 gcaaagaa
//
