LOCUS       40924_8446        5058 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-mCASP-1p10HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5058)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5058)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..276
                     /codon_start=1
                     /note="unnamed protein product; mCASP-1, p10 subunit"
                     /protein_id="SJL87735.1"
                     /translation="MGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEY
                     AWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH"
     CDS             286..312
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     polyA_signal    415..470
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(855..871)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(879..909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(923..944)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1002..1198
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1204..1338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1576..2164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2338..3195)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3196..3300)
                     /label=AmpR promoter
     enhancer        3331..3710
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3331..3708
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        3712..3989
                     /label=chicken beta-actin promoter
     intron          3990..5007
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tgggcattaa gaaggcccat atagagaaag attttattgc tttctgctct
       61 tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt cattgagtca
      121 ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat tttcagaaag
      181 gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc tgatagggtg
      241 accctgacaa aacgtttcta cctcttcccc ggacatagcg gccgctaccc atacgatgtt
      301 ccagattacg cttaatggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
      361 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
      421 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
      481 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
      541 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
      601 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
      661 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
      721 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
      781 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
      841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      901 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     1021 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1201 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1321 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
     1501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3301 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     3361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     3721 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     3781 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
     3841 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
     3901 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     3961 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
     4021 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     4081 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     4141 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     4201 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     4261 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     4321 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     4381 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     4441 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     4501 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     4561 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     4621 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     4681 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     4741 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     4801 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     4861 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     4921 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     4981 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     5041 catcattttg gcaaagaa
//
