LOCUS       40924_8441        5108 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-mCASP-1fa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..256)
                     /codon_start=1
                     /note="unnamed protein product; CASP1f"
                     /protein_id="SJL88365.1"
                     /translation="MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKL
                     ANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAG"
     misc_feature    complement(257..319)
                     /label=5UTR
                     /note="5UTR"
     polyA_signal    465..520
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(881..897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(905..921)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(929..959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(973..994)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1052..1248
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1254..1388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1626..2214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2388..3245)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3246..3350)
                     /label=AmpR promoter
     enhancer        3381..3760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3381..3758
                     /label=hCMV
                     /note="hCMV"
     promoter        3762..4039
                     /label=chicken beta-actin promoter
     intron          4040..5057
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctgccag gtagcagtct tcattacaaa tgtaagtgat aaagatttgg cttgcctggg
       61 gccctttttt agagacatga tcacataggt cccgtgcctt gtccatagca gtaatgtttg
      121 caagttttat tttatccatt tcttcctgat tcagcactct cttctctaaa agttcatcca
      181 acaatccatt tattgtccct atactcactg agttgataaa ttgcttcctc tttgccctca
      241 ggatcttgtc agccatggcg tttcttttct acaccgcaga gctactgaaa ctgaaagcat
      301 attgtgttgg cctgacgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
      361 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
      421 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
      481 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
      541 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
      601 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
      661 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
      721 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
      781 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
      841 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      901 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      961 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1021 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     1081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     1261 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1321 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1381 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1441 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     1561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1801 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2041 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3361 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     3421 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3481 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3541 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3601 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3661 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3721 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     3781 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     3841 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     3901 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     3961 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     4021 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     4081 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     4141 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     4201 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     4261 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     4321 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     4381 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     4441 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     4501 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     4561 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     4621 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     4681 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     4741 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     4801 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     4861 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     4921 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     4981 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     5041 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     5101 gcaaagaa
//
