LOCUS       V008816                 7171 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008816
VERSION     V008816
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    85..117
                     /label="5' UTR of mCASP-12"
                     /note="5' UTR of mCASP-12"
     CDS             118..1374
                     /gene="Casp12"
                     /label="Caspase-12"
                     /note="Caspase-12 from Mus musculus. Accession#: O08736"
     misc_feature    1378..2311
                     /label="3' UTR of mCASP-12"
                     /note="3' UTR of mCASP-12"
     polyA_signal    2528..2583
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2944..2960)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2968..2984)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2992..3022)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3036..3057)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3115..3311
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    3317..3451
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3689..4277)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4451..5308)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5309..5413)
                     /label="AmpR promoter"
     enhancer        5444..5823
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5444..5821
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5825..6102
                     /label="chicken beta-actin promoter"
     intron          6103..7120
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggatccccgg gaattcggca cgagggcacg agcggcacga gcggcacgag cacagccatg
      121 gcggccagga ggacacatga aagagatcca atctacaaga tcaaaggttt ggccaaggac
      181 atgctggatg gggtttttga tgacctggtg gagaagaatg ttttaaatgg agatgagtta
      241 ctcaaaatag gggaaagtgc gagtttcatc ctgaacaagg ctgagaatct ggttgagaac
      301 ttcttagaga aaacagacat ggcaggaaaa atatttgctg gccacattgc caattcccag
      361 gaacagctga gtttacaatt ttctaatgat gaggatgatg gacctcagaa gatatgtaca
      421 ccttcttctc catcagaatc caagagaaaa gtagaggatg atgaaatgga ggtaaatgct
      481 ggattggccc atgaatcaca tctaatgctg acagctcctc atggactcca gagctcagaa
      541 gtccaagata cactgaagct ttgtccacgt gatcagtttt gtaagataaa gacagaaagg
      601 gcaaaagaga tatatccagt gatggagaag gagggacgaa cacgtctggc tctcatcatc
      661 tgcaacaaaa agtttgacta cctttttgat agagataatg ctgatactga cattttgaac
      721 atgcaagaac tacttgaaaa tcttggatac tctgtggtgt taaaggaaaa ccttacagct
      781 caggaaatgg agacagagtt aatgcagttt gctggccgtc cagagcacca gtcctcagac
      841 agcacattcc tggtgtttat gtcccatggc atcctggaag gaatctgtgg ggtgaagcac
      901 cgaaacaaaa agccagatgt tcttcatgat gacactatct tcaaaatttt caacaactct
      961 aactgtcgga gtctgagaaa caaacccaag attctcatca tgcaggcctg cagaggcaga
     1021 tataatggaa ctatttgggt atccacaaac aaagggatag ccactgctga tacagatgag
     1081 gaacgtgtgt tgagctgtaa atggaataat agtataacaa aggcccatgt ggagacagat
     1141 ttcattgctt tcaaatcttc taccccacat aatatttctt ggaaggtagg caagactggt
     1201 tccctcttca tttccaaact cattgactgc ttcaaaaagt actgttggtg ttatcatttg
     1261 gaggaaattt ttcgaaaggt tcaacactca tttgaggtcc caggtgaact gacccagatg
     1321 cccactattg agagagtatc catgacacgc tatttctacc tttttcccgg gaattagcac
     1381 aggcaactct catgcagttc ccagtcaagt attgctgtag ctgagaagaa aagaaaattc
     1441 caagatccca ggatttttaa atgtgtaaaa cttttcagtt agatatttta tacaatatca
     1501 taaggctaga taatttccat aggttatttt taggggaaac ctgatacgta aaattttgca
     1561 tgaaaagtat agattcgttc cttccttcca aatctgctca tcttttactt tattttatta
     1621 aatttatatt tgcacatttt tgtaaaatga ataaaggaaa aagacactaa agaacatgtc
     1681 tgaacatatc ctggaacttc ttctttgtaa aatgaataaa ggaaaaagac actaaagaac
     1741 aaagatgata caccttttga tgaaccactt tcacctataa attagtagta agaatttact
     1801 cacaaggaag atcttatcta caatgtagct gagaaatgaa actgttgcat atgtaggtaa
     1861 aactaatcat cttttaggaa taagataaag aggtttatgt ccactgtttg tctctggcat
     1921 acatggtttt catgcctagt ggctacctac cattcctttc aatcaatttc ttaggatata
     1981 attgacaaat ttatgctatg tttataattt ttctcttaaa tgcctttgta ataagatata
     2041 ctcacacata cacatttaag attctgtcta ttagtgttat tgaaataaat cctagaaaca
     2101 aaaattaaaa gtttaaagca ttaaagacaa ttttgagaca aaatattcta tgaaattcta
     2161 tgcaaaattg aattcttcta caagtatgtg atgaagccca cctaccacta tttttgtcat
     2221 cttgaggatg ttatatcatt atttttactt aactgagctc aaattaaaat ctttgtttat
     2281 tctttgttct ataaaaaaaa aaaaaaaaaa actcgtgccg aattcgatct ctttttttgg
     2341 gtttggtggg gtatcttcat catcgaatag atagttatat acatcatcca ttgtagtggt
     2401 attaaacatc cctgtagtga ttccaaacgc gccaatgccc tggctcacaa ataccactga
     2461 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     2521 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     2581 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     2641 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     2701 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     2761 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     2821 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     2881 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     2941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3001 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt
     3061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     3121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3301 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3421 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3601 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5401 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
     5461 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     5521 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     5581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5641 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5701 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     5761 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5821 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     5881 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     5941 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa
     6001 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     6061 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc
     6121 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     6181 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     6241 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     6301 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     6361 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     6421 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     6481 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     6541 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     6601 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     6661 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     6721 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     6781 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     6841 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     6901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     6961 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     7021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     7081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     7141 tattgtgctg tctcatcatt ttggcaaaga a
//
