LOCUS       V008818                 6280 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008818
VERSION     V008818
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6280)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6280)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    80..105
                     /label="5' UTR of mCASP-11"
                     /note="5' UTR of mCASP-11"
     CDS             106..1224
                     /gene="Casp4"
                     /label="Caspase-4"
                     /note="Caspase-4 from Mus musculus. Accession#: P70343"
     misc_feature    1228..1420
                     /label="3' UTR of mCASP-11"
                     /note="3' UTR of mCASP-11"
     polyA_signal    1637..1692
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2053..2069)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2077..2093)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2101..2131)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2145..2166)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2224..2420
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2426..2560
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2798..3386)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3560..4417)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4418..4522)
                     /label="AmpR promoter"
     enhancer        4553..4932
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4553..4930
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4934..5211
                     /label="chicken beta-actin promoter"
     intron          5212..6229
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggggaattcg gcacgagcgg tgcgaaagaa ctgaggcttt ttctcatggc tgaaaacaaa
      121 caccctgaca aaccacttaa ggtgttggaa cagctgggca aagaagtcct tacggagtac
      181 ctagaaaaat tagtacaaag caatgtactg aaattaaagg aggaagataa acaaaaattt
      241 aacaatgctg aacgcagtga caagcgttgg gtttttgtag atgccatgaa aaagaaacac
      301 agcaaagtag gtgaaatgct tctccagaca ttcttcagtg tggacccagg cagccaccat
      361 ggtgaagcta atctggaaat ggaggaacca gaagaatcat tgaacactct caagctttgt
      421 tcccctgaag agttcacaag gctttgcaga gaaaagacac aagaaattta cccaataaag
      481 gaggccaatg gccgtacacg aaaggctctt atcatatgca atacagagtt caaacatctc
      541 tcactgaggt atggggctaa ctttgacatc attggtatga aaggccttct tgaagactta
      601 ggctacgatg tggtggtgaa agaggagctt acagcagagg gcatggagtc agagatgaaa
      661 gactttgctg cactctcaga acaccagaca tcagacagca cattcctggt gctaatgtct
      721 catggcacac tgcatggcat ttgtggaaca atgcacagtg aaaaaactcc agatgtgcta
      781 cagtatgata ccatctatca gatattcaac aattgccact gtccaggtct acgagacaaa
      841 cccaaagtca tcattgtgca ggcctgcaga ggtgggaact ctggagaaat gtggatcaga
      901 gagtcttcaa aaccccagtt gtgcagaggt gtagatctac ctaggaatat ggaagctgat
      961 gctgtcaagc tgagccacgt ggagaaggac ttcattgcct tctactctac aaccccacat
     1021 cacttgtcct accgagacaa aacaggaggc tcttacttca tcactagact catttcctgc
     1081 ttccggaaac atgcttgctc ttgtcatctc tttgatatat tcctgaaggt gcaacaatca
     1141 tttgaaaagg caagtattca ttcccagatg cccaccattg atcgggcaac cttgacgaga
     1201 tatttctacc tctttcctgg caactgagaa caaagcaaca agcaactgaa tctcatttct
     1261 tcagcttgaa gaagtgatct tggccaagga tcacattcta ttcctgaaat tccagaacta
     1321 gtgaaattaa ggaaagaata cttatgaatt caagaccagc ctaagcaaca cagtgggatt
     1381 ctgttccata gacaagcaaa caagcaaaaa taaaaaaaaa ctcgtgccga attcgatctc
     1441 tttttttggg tttggtgggg tatcttcatc atcgaataga tagttatata catcatccat
     1501 tgtagtggta ttaaacatcc ctgtagtgat tccaaacgcg ccaatgccct ggctcacaaa
     1561 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     1621 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1681 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1741 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1801 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1861 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1921 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1981 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2101 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     2221 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2281 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2401 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     2461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     2581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2701 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4561 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4621 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4681 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4741 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4801 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4861 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4921 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4981 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     5041 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     5101 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     5161 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     5221 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     5281 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     5341 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     5401 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     5461 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     5521 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     5581 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     5641 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5701 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5761 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5821 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5881 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5941 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     6001 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     6061 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     6121 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     6181 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     6241 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//
