LOCUS       V008819                 6308 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008819
VERSION     V008819
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..118
                     /label="5' UTR of mCASP-1"
                     /note="5' UTR of mCASP-1"
     CDS             119..1324
                     /gene="Casp1"
                     /label="Caspase-1"
                     /note="Caspase-1 from Mus musculus. Accession#: P29452"
     misc_feature    1328..1438
                     /label="3' UTR of mCASP-1"
                     /note="3' UTR of mCASP-1"
     polyA_signal    1665..1720
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2081..2097)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2105..2121)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2129..2159)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2173..2194)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2252..2448
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2454..2588
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2826..3414)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3588..4445)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4446..4550)
                     /label="AmpR promoter"
     enhancer        4581..4960
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4581..4958
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4962..5239
                     /label="chicken beta-actin promoter"
     intron          5240..6257
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaag cttggccaaa acggccgagc tcgaattcgt ccggccgagc tcgaattcgt
       61 caggccaaca caatatgctt tcagtttcag tagctctgcg gtgtagaaaa gaaacgccat
      121 ggctgacaag atcctgaggg caaagaggaa gcaatttatc aactcagtga gtatagggac
      181 aataaatgga ttgttggatg aacttttaga gaagagagtg ctgaatcagg aagaaatgga
      241 taaaataaaa cttgcaaaca ttactgctat ggacaaggca cgggacctat gtgatcatgt
      301 ctctaaaaaa gggccccagg caagccaaat ctttatcact tacatttgta atgaagactg
      361 ctacctggca ggaattctgg agcttcaatc agctccatca gctgaaacat ttgttgctac
      421 agaagattct aaaggaggac atccttcatc ctcagaaaca aaggaagaac agaacaaaga
      481 agatggcaca tttccaggac tgactgggac cctcaagttt tgccctttag aaaaagccca
      541 gaagttatgg aaagaaaatc cttcagagat ttatccaata atgaatacaa ccactcgtac
      601 acgtcttgcc ctcattatct gcaacacaga gtttcaacat ctttctccga gggttggagc
      661 tcaagttgac ctcagagaaa tgaagttgct gctggaggat ctggggtata ccgtgaaagt
      721 gaaagaaaat ctcacagctc tggagatggt gaaagaggtg aaagaatttg ctgcctgccc
      781 agagcacaag acttctgaca gtactttcct tgtattcatg tctcatggta tccaggaggg
      841 aatatgtggg accacatact ctaatgaagt ttcagatatt ttaaaggttg acacaatctt
      901 tcagatgatg aacactttga agtgcccaag cttgaaagac aagcccaagg tgatcattat
      961 tcaggcatgc cgtggagaga aacaaggagt ggtgttgtta aaagattcag taagagactc
     1021 tgaagaggat ttcttaacgg atgcaatttt tgaagatgat ggcattaaga aggcccatat
     1081 agagaaagat tttattgctt tctgctcttc aacaccagat aatgtgtctt ggagacatcc
     1141 tgtcaggggc tcacttttca ttgagtcact catcaaacac atgaaagaat atgcctggtc
     1201 ttgtgacttg gaggacattt tcagaaaggt tcgattttca tttgaacaac cagaatttag
     1261 gctacagatg cccactgctg atagggtgac cctgacaaaa cgtttctacc tcttcccggg
     1321 acattaaacg aagaatccag ttcattctta tgtacctatg ctgagaatcg tgccaataag
     1381 aagccaatac ttccttagat gatgcaataa atattaaaat aaaacaaaac agaaggctaa
     1441 aaaaaaaaaa aaaaaagctc gcccggggat cctctaggcg gccgctaggc ctttttggcc
     1501 aagctcgaat ttcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     1561 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     1621 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     1681 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     1741 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     1801 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     1861 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     1921 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     1981 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2041 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2161 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2221 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     2281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2401 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     2461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2581 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4561 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     4621 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4681 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4741 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4801 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4861 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4921 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     4981 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     5041 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     5101 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     5161 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     5221 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     5281 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     5341 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     5401 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     5461 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     5521 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     5581 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     5641 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     5701 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     5761 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     5821 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     5881 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     5941 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     6001 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     6061 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     6121 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     6181 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     6241 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     6301 gcaaagaa
//
